Povezanost funkcionalnog polimorfizma gena za GluR7 podjedinicu ionotropnog glutamatnog receptora (GRIK) i razvoja ovisnosti o alkoholu

master's thesis

• Title (english): Ser310Ala functional polymorphism in the GluR7 ionotropic glutamate receptor subunit gene (GRIK3) and development of alcohol dependence
• Author: Boris Kuzman
• Mentor: Dubravka Švob Štrac (mentor)
• Mentor: Domagoj Đikić (mentor)
• Committee member: Domagoj Đikić (predsjednik povjerenstva)
• Committee member: Dubravka Švob Štrac (član povjerenstva)
• Committee member: Inga Marijanović (član povjerenstva)
• Committee member: Mladen Kučinić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-12-06, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Postoje brojni dokazi o uključenosti glutamata u akutne i kronične učinke alkohola, te pojavu ovisnosti i sindroma ustezanja, a glutamatni sustav predstavlja potencijalni cilj u liječenju alkoholizma. Nedavna istraživanja uputila su na povezanost alkoholizma i gena koji kodiraju podjedinice ionotropnih glutamatnih receptora. Cilj istraživanja bio je utvrditi postoji li u ispitanika hrvatskog podrijetla povezanost funkcionalnog polimorfizma Ser310Ala (rs6691840) smještenog u genu GRIK3, koji kodira za GluR7 podjedinicu glutamatnih kainatnih receptora, s rizikom razvoja ovisnosti o alkoholu. U tu svrhu u istraživanje je uključeno 275 ispitanika ovisnih o alkoholu, te 208 zdravih dobrovoljaca. U sklopu istraživanja željeli smo istražiti i moguće razlike u raspodjeli GRIK3 genotipova i alela između skupina ispitanika različitog spola i pušačkog statusa, te u ispitanika ovisnih o alkoholu podijeljenih s obzirom na suicidalno i agresivno ponašanje, početak zlouporabe alkohola, kao i na tip alkoholizma prema Cloningerovoj klasifikaciji. Rezultati pokazuju manju učestalost homozigotnog CC genotipa u ispitanika koji su počeli zloupotrebljavati alkohol prije 25. godine života, u odnosu na alkoholičare s kasnijim početkom zlouporabe alkohola. Daljnja istraživanja potrebna su daljnja istraživanja kako bi se razjasnila uloga gena GRIK3 u alkoholizmu.

Abstract (english)

There is accumulating data suggesting an involvement of glutamate in the acute and chronic effects of alcohol, including dependence and withdrawal, and glutamatergic system is emerging as potential target for alcoholism treatment. Recent studies have suggested an association of ionotropic glutamate receptor genes with alcohol dependence. The aim of this study was to examine the association of Ser310Ala functional polymorphism (rs6691840) in the GluR7 glutamate kainate receptor subunit gene (GRIK3) with the development of alcoholism. The study included 275 alcohol-dependent patients and 208 healthy subjects. Possible differences in the frequency of GRIK3 genotypes and alleles between subjects of different gender and smoking status, as well as between alcohol-dependent patients stratified according to aggressive and suicidal behavior, age of drinking onset, and type of alcoholism according to Cloninger’s classification were also studied. The results demonstrated lower frequency of homozygous CC genotype in patients who started abusing alcohol before 25. years of age in relation to alcohol-dependent subjects with the late onset of alcohol abuse. Further studies are needed to elucidate the role of GRIK3 gene in the development of alcohol dependence.

• Keywords: alkoholizam; glutamatni kainatni receptor; gen GRIK3; polimorfizam; rs6691840
• Keywords (english): alcoholism; glutamate kainate receptor; GRIK3 gene; polymorphism; rs6691840
• Language: croatian
• URN:NBN: urn:nbn:hr:217:463831
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-27 09:57:20