Genotoksični učinak zlatnih i srebrnih nanočestica na gujavicu Eisenia fetida (Savigny, 1826)

master's thesis

• Title (english): Genotoxic effect of gold and silver nanoparticles on earthworm Eisenia fetida
• Author: Damir Lisjak
• Mentor: Goran Klobučar (mentor)
• Committee member: Goran Klobučar (predsjednik povjerenstva)
• Committee member: Mirjana Pavlica (član povjerenstva)
• Committee member: Sandra Radić Brkanac (član povjerenstva)
• Committee member: Ivana Maguire (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-09-23, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U posljednjih nekoliko godina upotreba nanotehnologije je u velikom porastu, uključujući i mnoge nove materijale koji sadrže nanočestice. Nanočestice definiramo kao materijale koji su u barem jednoj dimenziji manji od 100 nm. Njihovo biološko djelovanje još nije u potpunosti poznato. Zbog malih dimenzija, ali jako velike površine nanočestice mogu inducirati oksidacijski stres i djelovati genotoksično. U ovom radu mjereno je genotoksično djelovanje zlatnih i srebrnih nanočestica na stanicama celomocita gujavica (Eisenia fetida, Savigny 1826) pomoću alkalnog komet testa. Za procjenu genotoksičnog utjecaja nanočestica određenog metala važno je dobiti uvid i u genotoksično djelovanje ionskog oblika tog istog metala, pa se u tu svrhu mjerila i genotoksičnost soli srebra i zlata (AgNO3, KAuCl4). Izlaganje je trajalo 10 dana, a provedeno je u kontroliranim laboratorijskim uvjetima u svibnju 2012. god. Oštećenja DNA pokazuju da su ionski oblici zlata i srebra štetniji od nanočestica istih spojeva.

Abstract (english)

In the recent years use of nanotechnology has increased, including many new materials containing nanoparticles. Nanoparticles are defined as materials smaller than 100 nm, in one dimension at least. Their biological activity is not yet fully known. Due to their small size, but large surface area, nanoparticles can induce oxidative stress and genotoxic effect. In this study we have measured the genotoxic influence of gold and silver nanoparticles on earthworms' coelomocytes (Eisenia fetida, Savigny 1826) using alkaline comet assay. For the assessment of genotoxic effects of nanoparticles of particular metal, it is important to gain insight into the genotoxicity of its ionic forms. For this purpose genotoxicity of silver and gold salts (AgNO3, KAuCl4) was also measured. Exposure lasted 10 days and it was conducted under controlled laboratory conditions in May 2012. DNA damage showed that ionic forms of gold and silver nanoparticles are more harmful than nanoparticles in elemental state.

• Keywords: Eisenia fetida; celomociti; komet-test; nanočestice; zlato; srebro
• Keywords (english): Eisenia fetida; coelomocytes; comet-assay; nanoparticles; gold; silver
• Language: croatian
• URN:NBN: urn:nbn:hr:217:238006
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-27 10:00:00