Genska struktura i raznolikost potočne mrene (Barbus balcanicus Kotlík, Tsigenopoulos, Ráb & Berrebi, 2002; Actinopteri) u Hrvatskoj

master's thesis

• Title (english): Genetic structure and variety of the danube barbel (Barbus balcanicus Kotlík, Tsigenopoulos, Ráb &Berrebi, 2002; Actinopteri) in Croatia
• Author: Vatroslav Veble
• Mentor: Ivana Buj (mentor)
• Committee member: Ivan Čanjevac (predsjednik povjerenstva)
• Committee member: Blanka Cvetko Tešović (član povjerenstva)
• Committee member: Ivana Buj (član povjerenstva)
• Committee member: Ivan Radosavljević (član povjerenstva)
• Committee member: Zoran Marčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2020-02-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Potočna mrena (Barbus balcanicus Kotlík, Tsigenopoulos, Ráb i Berrebi, 2002) je slatkovodna riba iz porodice Cyprinidae čiji srodstveni odnosi među populacijama u Hrvatskoj nisu poznati, kao ni protok gena između tih populacija, niti je utvrđen čitav areal njene rasprostranjenosti u Hrvatskoj. Zbog onečišćenja vodotoka, nestajanja njenih prirodnih i mrijesnih staništa i regulacije gornjih tokova rijeka, potočna mrena je na popisu osjetljivih vrsta slatkovodnih riba Hrvatske. Kako bih predložio prikladne mjere zaštite te pridonio istraženosti potočne mrene na području Hrvatske, proveo sam filogenetsku rekonstrukciju na temelju sekvenci mitohondrijskog gena za citokrom b pomoću metode najveće parsimonije, metode najveće vjerojatnosti i metode susjednog sparivanja. Pomoću programa DnaSP odredio sam mjere genskog polimorfizma, a stope migracija između populacija odredio sam pomoću programa Migrate. Rezultati filogenetske rekonstrukcije pokazali su da je potočna mrena dobro strukturirana vrsta te da postoje tri linije potočne mrene, od kojih je samo jedna s područja Hrvatske. Utvrđena je različita intrapopulacijska raznolikost, a migracije između populacija nisu recipročne.

Abstract (english)

The Danube barbel (Barbus balcanicus Kotlík, Tsigenopoulos, Ráb & Berrebi, 2002) is a freshwater fish from the Cyprinidae family, whose relationships between populations in Croatia are unknown, as well as gene flow between those population, and the entire range of its distribution in Croatia has not been established. Due to the pollution of watercourses, the disappearance of its natural and spawning habitats and the regulation of the upper river flows, the Danube barbel is listed as a vulnerable species of freshwater fish in Croatia. In order to propose suitable protection measures and to contribute to the study of the Danube barbel in Croatia, I performed phylogenetic reconstruction based on the cytochrome b mitochondrial gene sequences using the maximum parsimony method, the maximum likelihood method and the neighbor joining method. Using the DnaSP computer program, I determined the measures of gene polymorphism, and I determined the migration rates between populations using the Migrate program. The results of the phylogenetic reconstruction showed that the Danube barbel is a well structured species and that there are three lineages of the Danube barbel, only one of which is from the territory of Croatia. Different intrapopulation diversity was identified and migrations between populations were not reciprocal.

• Keywords: slatkovodne ribe; filogenija; citokrom b; protok gena; zaštita
• Keywords (english): freshwater fish; phylogeny; cytochrome b; gene flow; protection
• Language: croatian
• URN:NBN: urn:nbn:hr:217:326416
• Study programme: Title: Enviromental Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-02-27
• Created on: 2021-02-23 08:23:54