Učinak sustavne hipertermije na antitumorski potencijal cisplatine i nanokristala resveratrola kod Ehrlichovog ascitesnog tumora u miša

master's thesis

• Title (english): Effect of systemic hyperthermia on the antitumor potential of cisplatin and resveratrol nanocrystals on Ehrlich ascites solid tumor in mouse
• Author: Dragica Marinčić
• Mentor: Nada Oršolić (mentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Martina Šeruga Musić (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Committee member: Sofia Ana Blažević (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-02-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Cilj ovog istraživanja bio je istražiti sinergistički učinak sustavne hipertermije uz nanokristale resveratrola i cisplatinu na inhibiciju brzorastućeg EAT, preživljavanje miševa, polarizaciju makrofaga te sposobnost nanokristala resveratrola u smanjenju toksičnih učinaka cisplatine i hipertermije u jetri i bubregu miševa. Miševima soja Swiss albino izazvan je tumor injiciranjem 1x106 stanica EAT subkutano u bedro, a zatim su obrađeni injiciranjem nanokristala resveratrola u dozi 50 mg/kg per os tijekom 5 dana u nizu te cisplatine u dozi 2,5 mg/kg 10. i 12. dan te u dozi 5 mg/kg 14. dan uz izlaganje sustavnoj hipertermiji pri 41°C. Dobiveni rezultati pokazuju da samostalna primjena nanokristala resveratrola i izlaganje hipertermiji vodi rastu tumora dok primjena nanokristala resveratrola i cisplatine uz izlaganje sustavnoj hipertermiji ostvaruje izraziti antitumorski učinak inhibicijom rasta EAT u solidnom obliku, ali ne pridonosi znatnijem povećanju preživljavanja životinja. Injiciranje cisplatine pri hipertermalnim uvjetima povećava životni vijek miševa za 47% u odnosu na kontrolu. Temeljem dobivenih rezultata možemo zaključiti da nanokristali resveratrola mogu polučiti antioksidacije i/ili prooksidacijske učinke u različitim mikrookolišnim uvjetima, ali i sudjelovati u povećanju makrofagne plastičnosti i polarizacije te zaštititi bubreg i jetru od toksičnosti izazvane cisplatinom.

Abstract (english)

The aim of this study was to investigate the synergistic effect of systemic hyperthermia with resveratrol nanocrystals and cisplatin on the inhibition of fast-growing EAT, mouse survival, macrophage polarization, and the ability of resveratrol nanocrystals to reduce the toxic effects of cisplatin and hyperthermia. In Swiss albino mice tumor were induced by injecting 1x106 EAT cells subcutaneously into the thigh and then treated by injecting resveratrol nanocrystals at a dose of 50 mg/kg per os for 5 consecutive days and cisplatin at a dose of 2,5 mg/kg day 10 and 12 and at a dose of 5 mg/kg on day 14 with exposure to systemic hyperthermia at 41°C. The results show that the use of resveratrol nanocrystals alone and exposure to hyperthermia leads to tumor growth, while the use of resveratrol and cisplatin nanocrystals with exposure to systemic hyperthermia has a pronounced antitumor effect by inhibiting EAT growth in solid form, but does not significantly increase animal survival. Injection of cisplatin under hyperthermal conditions increased the lifespan of mice by 47% compared to control. Based on the obtained results, we can conclude that resveratrol nanocrystals have antioxidant and/or prooxidative effects in various microenvironmental conditions, but also participate in increasing macrophage plasticity and polarization and protect the kidney and liver from cisplatin-induced toxicity.

• Keywords: sustavna hipertermija; cisplatina; nanotehnologija; resveratrol; solidni tumor; oksidacijski stres
• Keywords (english): sistemic hyperthermia; cisplatin; nanotehnology; resveratrol; solid tumor; oxidative stress
• Language: croatian
• URN:NBN: urn:nbn:hr:217:171428
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-02-26
• Created on: 2021-03-09 08:01:31