Utjecaj solnog i osmotskog stresa na diferencijalnu ekspresiju proteina kaktusa Mammillaria gracilis Pfeiff. u kulturi in vitro

master's thesis

• Title (english): Effects of salinity and osmotic stress on protein differential expression in Mammillaria gracilis Pfeiff. in vitro-grown cultures
• Author: Tea Rogić
• Mentor: Biljana Balen (mentor)
• Mentor: Mario Cindrić (mentor)
• Committee member: Biljana Balen (predsjednik povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Committee member: Mario Cindrić (član povjerenstva)
• Committee member: Inga Marijanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-10-30, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Iako kaktus Mammillaria gracilis Pfeiff. nije komercijalno zanimljiva biljna vrsta u Europi, njegove prilagodbe na suha staništa čine ga prikladnim modelnim organizmom za proučavanje biokemijskih i molekularnih mehanizama koji se aktiviraju tijekom izlaganja biljaka solnom i osmotskom stresu. Tkiva kaktusa u kulturi in vitro rastu u obliku habituiranog neorganiziranog kalusa, koji zadržava regeneracijski potencijal, te u obliku potpuno neorganiziranog tumora dobivenog transformacijom stanica kaktusa bakterijom Agrobacterium tumefaciens. Ovim istraživanjem pokazano je da kalusno i tumorsko tkivo reagiraju na prisutnost solnog i osmotskog stresa na razini ekspresije topivih staničnih proteina. Mehanizmi uključeni u odgovore biljke na solni i osmotski stres razlikuju se i ovise o vrsti ispitivanog tkiva, ali čini se da je tumorsko tkivo jače pogođeno stresom od kalusnog tkiva. Razlikovna analiza ekspresije proteina u ispitivanim tkivima ukazala je na proteine toplinskog šoka i gliceraldehid-3-fosfat dehidrogenazu kao potencijalne opće biljege prisutnosti solnog i osmotskog stresa kod biljaka.

Abstract (english)

Although cacti Mammillaria gracilis Pfeiff. is not commercially interesting plant species in Europe, its adaptations on arid environments make it suitable model organism for studying biochemical and molecular mechanisms which activate during exposure of plants to salt and osmotic stress. Cacti tissues grow in vitro as habituated calli of unorganised growth capable of regeneration potential retention, and as completely unorganised tumors induced via transformation of cacti cells with Agrobacterium tumefaciens bacteria. This research showed that calli and tumor tissues respond to salt and osmotic stress at the level of soluble cell proteins synthesis. Mechanisms underlying these responses differ and are dependent of tissue used, but it seems that tumor tissue is, at least to some extent, more sensitive to impact of stress factors than calli. Differential expression protein analysis implicated heat shock protein family, as well as glyceraldehyde-3-phosphate dehydrogenase protein, as general markers of salt and osmotic stress presence in plants.

• Keywords: kaktus; solni stres; osmotski stres; 2D elektroforeza; spektrometrija masa
• Keywords (english): cactus; salt stress; osmotic stress; 2D electrophoresis; mass spectrometry
• Language: croatian
• URN:NBN: urn:nbn:hr:217:516689
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-28 07:55:50