Uloga krizina na histomorfometrijske značajke osteoporoze prouzročene retinoičnom kiselinom u štakora

master's thesis

• Title (english): The role of chrysin on the histomorphometrical characteristics of osteoporosis caused by retinoic acid in rats
• Author: Kristijan Sašilo
• Mentor: Nada Oršolić (mentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Mirjana Kalafatić (član povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-09-25, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Oksidativni stres nastaje uslijed neravnoteže između povećane proizvodnje reaktivnih kisikovih radikala i nedovoljne aktivnosti antioksidativnih sustava. Uloga radikala utvrđena je u patogenezi brojnih bolesti, uključujući i osteoporozu. Lipidna peroksidacija jedna je od glavnih karakteristika oksidativnog stresa, a malondialdehid (MDA) jedan je od njenih krajnjih proizvoda te mjera proizvodnje slobodnih radikala. Pokazano je da med (pčelinji proizvod bogat antioksidansom krizinom) ima značajan učinak u sprječavanju osteoporoze stoga smo istražili učinak krizina na težinu, histomorfometrijske parametre kostiju, udio kalcija i fosfora te koncentracije MDA i glutationa (GSH) pokusnih životinja. Na temelju rezultata zaključili smo da krizin ima značajnu antioksidativnu i antiosteoporotsku aktivnost te da bi njegova primjena mogla poslužiti u sprječavanju osteoporoze i razumijevanju odnosa između funkcionalne prehrane i zdravlja.

Abstract (english)

Disbalance between the increased production of reactive oxygen species and the inadequate activity of the antioxidant system causes oxidative stress. Role of the free radical in pathogenesis of many diseases, including osteoporosis, has been confirmed. Lipid peroxidation is one of the main characteristics of the oxidative stress, and malondialdehyde (MDA) one of its ultimate products, as well as the indicator of free radical production. It has been shown that bee honey (a natural product rich in antioxidant chrysin) has significant effect on prevention of osteoporosis, therefore we decided to investigate the effects of chrysin on body weight, histomorphometrical parameters of bone, calcium and phosphorus contents, as well as on concentrations of MDA and glutathione (GSH) in test animals. Based on the obtained results, we concluded that chrysin displays significant antioxidative and antiosteoporotic activity and that its use could be helpful in preventing osteoporosis and understanding the relationship between the functional diet and health.

• Keywords: osteoporoza; oksidativni stres; lipidna peroksidacija; glutation; histomorfometrijska analiza
• Keywords (english): osteoporosis; oxidative stress; lipid peroxidation; glutathione; histomorphometrical analysis
• Language: croatian
• URN:NBN: urn:nbn:hr:217:657670
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-28 08:07:09