Primjena proteomske analize u identifikaciji biomarkera karcinoma prostate

master's thesis

• Title (english): Application of proteomic analysis in the identification of prostate carcinoma biomarkers
• Author: Anamarija Skenderović
• Mentor: Mario Cindrić (mentor)
• Committee member: Mario Cindrić (predsjednik povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Committee member: Mirna Ćurković Perica (član povjerenstva)
• Committee member: Inga Marijanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-02-15, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Proteomska tehnologija u istraživanju tumorskih bolesti koristi se u detekciji ranih stadija bolesti, određivanju stadija i progresije bolesti, otkrivanja potencijalnih meta i biomarkera za liječenje bolesti te u konačnici za primjenu terapije prilagođene svakom individualnom pacijentu na osnovu njegovog proteomskog profila. Tumor prostate jedno je od najčešće dijagnosticiranih malignih oboljenja muškaraca nakon pedesete godine života. U ovom radu primijenjen je proteomski pristup za utvrđivanje razlikovnog prikaza oboljenja karcinoma prostate, a u svrhu pronalaska potencijalnih novih biomarkera za bolju procjenu progresije i agresivnosti bolesti. Nakon histopatološkog nalaza, proteini tumorom zaraženog i zdravog tkiva prostate identificirani su dvodimenzionalnom gel elektroforezom u sprezi sa spektrometrijom masa. Dobiveni rezultati i razvijena metodologija čine okosnicu za daljnja istraživanja na većem broju uzoraka.

Abstract (english)

Proteomics technology in the research of tumor diseases is used in detection of early stages of disease development, tumor staging and progression, identification of potential targets and biomarkers. Collected proteomics data could direct disease treatment and could have impact on administrated therapy adjusted for every individual patient based on his proteomics profile. Prostate tumor is one of the leading diagnosed malignant disease of men after the age of 50. Proteomics methodology applied in the thesis utilitized for an observation of differential display of prostate tumor disease vs. health disease. Final goal was identification of potential novel biomarkers for better understanding of disease progression and agression. After histopathological determination, proteins from tumor-infected and health prostate tissue were separated by two-dimensional gel electrophoresis followed by mass spectrometry identification. Presented results and methodology can be used as basis for following population researches.

• Keywords: tumor prostate; proteomika; dvodimenzionalna gel elektroforeza; spektrometrija masa; biomarkeri tumora prostate
• Keywords (english): prostate tumor; proteomics; two-dimensional gel electrophoresis; mass spectrometry; prostate cancer biomarkers
• Language: croatian
• URN:NBN: urn:nbn:hr:217:703069
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-28 08:10:08