Ispitivanje citotoksičnosti novih spojeva diazenkarboksamida kao potencijalnih citostatika

master's thesis

• Title (english): Cytotoxicity testing of new compounds diazencarboxamides as potential cytostatics
• Author: Sanja Soviček
• Mentor: Maja Osmak (mentor)
• Mentor: Domagoj Đikić (mentor)
• Committee member: Domagoj Đikić (predsjednik povjerenstva)
• Committee member: Maja Osmak (član povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2013-09-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Nedovoljna učinkovitost postojećih terapija potiče traganje za novim, učinkovitijim lijekovima za pacijente oboljele od tumora, uključivši nove citotoksične spojeve. Kako bi se poboljšala svojstva diazenkarboksamida, sintetizirani su novi spojevi, diazendikarboksamidi. Tema ovog diplomskog rada bila je ispitati njihovu biološku aktivnost. Prema rezultatima MTT testa dobivenih na stanicama karcinoma vrata maternice HeLa, najučinkovitiji je bio spoj JV-158. Njegova citotoksičnost ispitana je na više različitih linija tumorskih stanica, te pokazala da su najosjetljivije HeLa stanice, a najmanje osjetljive stanice karcinoma debelog crijeva HCT-116. Iscrpljivanje unutarstanične koncentracije glutationa nije utjecalo na preživljenje stanica obrađenih sa spojem JV-158, što ukazuje da glutation ne sudjeluje u staničnom odgovoru. Ispitivanje vrste stanične smrti koju potiče JV-158 je pokazalo da se radi o nekrozi. Na temelju dobivenih rezultata možemo pretpostaviti kako bi spoj JV-158 mogao biti novi potencijalni protutumorski lijek.

Abstract (english)

Insufficient effectiveness of current therapy of cancer patients has stimulated the search for new, more effective tretament, including the screening of new compounds. To increasae the beneficial characteristics of diazencarboxamides, new compounds, diazendicarboxamides have been synthesised. The theme of this graduation thesis was to investigate their biological activity. The most effective among them was JV-158, as determined by MTT assay on cervical carcinoma HeLa cells. Its cytotoxicity was tested further on several tumor cells lines showing that Hela cells were the most sensitive, while colon carcinoma HCT-116 cells were most resistant. Depletion of intracellular glutathione level did not change the sensitivity of HeLa cells to JV-158, indicating that GSH was not involved in cell response to this compound. Regarding the type of induced cell death, JV-158 triggers necrosis. Based on these results we can assume that the compound JV-158 could be a new potential anti-cancer drug.

• Keywords: diazendikarboskamidi; citotoksičnost; protutumorski spojevi
• Keywords (english): diazenedicarboxamides; cytotoxicity; anti-cancer compounds
• Language: croatian
• URN:NBN: urn:nbn:hr:217:782016
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-28 08:18:31