Populacijska struktura i raznolikost oštrulje (Aulopyge huegelii Heckel, 1943; Cypriniformes, Actinopteri) u Hrvatskoj i Bosni i Hercegovini

master's thesis

• Title (english): Population structure and diversity of Dalmatian barbelgudgeon (Aulopyge huegelii Heckel, 1943; Cypriniformes, Actinopteri) in Croatia and Bosnia and Herzegovina
• Author: Mia Knjaz
• Mentor: Ivana Buj (mentor)
• Committee member: Ivana Buj (predsjednik povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Committee member: Sunčica Bosak (član povjerenstva)
• Committee member: Zoran Marčić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2020-09-25, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Aulopyge huegelli Heckel, 1943 (Cypriniformes, Actinopteri) ili oštrulja endem je vodotoka
Jadranskog slijeva. Naseljava krške rijeke i jezera u Hrvatskoj i Bosni i Hercegovini. Zbog malog i
rascjepkanog areala oštrulja se smatra ugroženom na lokalnoj i globalnoj razini. Istraživanja na ovoj
vrsti su malobrojna, a molekularni podaci oskudni. Cilj ovog istraživanja bio je pomoći opisivanju
strukture populacija oštrulje u Hrvatskoj i Bosni i Hercegovini analizom gena za citokrom b i
mitohondrijske kontrolne regije. Uzorci su prikupljeni na lokalitetima diljem cijelog areala
rasprostranjenosti ove vrste. U laboratoriju je izolirana ukupna genomska DNA, a lančanom
reakcijom polimerazom umnožen je gen citokrom b i dio mitohondrijske kontrolne regije.
Uspješnost reakcija provjerena je elektroforezom na agaroznom gelu, a na uspješno izoliranim
uzorcima izvršeno je sekvenciranje. Filogenetske rekonstrukcije populacija provedene su
metodama maksimalne parsimonije, najveće vjerojatnosti te susjednog sparivanja. Metodom
Bayesove inferencije procijenjen je protok gena između populacija te efektivne veličine populacija.
Rezultati pokazuju prisutnost 13 haplotipova citokroma b i 11 haplotipova kontrolne regije na
istraživanim lokalitetima. Vrsta pokazuje umjerenu genetičku raznolikost i slabu genetičku
strukturiranost. Protok gena između populacija nije ustanovljen. Efektivne veličine populacija su
male. Kao evolucijski značajne jedinice utvrđene su populacije na lokalitetima Roški slap na Krki,
Ruda na Cetini te rijeka Šujica i vodotoci oko Tomislavgrada u Duvanjskom i Livanjskom polju.

Abstract (english)

Aulopyge huegelli Heckel, 1943 (Cypriniformes, Actinopterygii) or dalmation barbelgudgeon is
endemic to the Adriatic Basin. It inhabits karst rivers and lakes in Croatia and Bosnia and
Herzegovina. Due to its small and fragmented distribution area it is considered endangered
locally and globally. Research on this species is limited, and molecular data are scarce. The aim
of this study was to help describe the structure of dalmation barbelgudgeon populations in
Croatia and Bosnia and Herzegovina by analysing genes for cytochrome b and mitochondrial
control region. Samples were collected at sites along the entire distribution area of this
species. Total genomic DNA was isolated in the laboratory, the cytochrome b gene and part of
the mitochondrial control region were amplified by polymerase chain reaction. The success of
the reactions was checked by agarose gel electrophoresis, and sequencing was performed on
successfully isolated samples. Phylogenetic reconstructions of populations were obtained by
the methods of maximum parsimony, maximum likelihood, and median joining. Bayesian
inference method was used to estimate gene flow between populations and the effective size
of populations. The results show the presence of 13 cytochrome b haplotypes and 11 control
region haplotypes at research sites. The species shows moderate genetic diversity and weak
genetic structuring. Gene flow between populations has not been detected. Effective
population sizes are small. The populations of Roški slap on the Krka river, Ruda on the Cetina
river and Šujica river with the watercourses around Tomislavgrad in Duvanjsko and Livanjsko
fields have been identified as evolutionarily significant units.

• Keywords: oštrulja; endem; molekularna filogenija; populacijska genetika; konzervacijski prioriteti
• Keywords (english): Dalmation barbelgudgeon; endemic species; molecular phylogeny; population genetics; conservation priorities
• Language: croatian
• URN:NBN: urn:nbn:hr:217:219982
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2022-09-25
• Created on: 2021-04-26 12:35:43