Učinak virusa uvijenosti lista vinove loze 3 na indikatore stresa u vinovoj lozi

master's thesis

• Title (english): Influence of grapevine leafroll-associated virus 3 on stress indicators in grapevine
• Author: Emanuel Gaši
• Mentor: Sandra Radić Brkanac (mentor)
• Committee member: Sandra Radić Brkanac (predsjednik povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Kristina Pikelj (član povjerenstva)
• Committee member: Ivan Čanjevac (član povjerenstva)
• Committee member: Alan Moro (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-05-13, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Cilj ovog rada bio je istražiti učinak sojeva virusa GLRaV-3 i njihovih kombinacija na parametre oksidacijskog stresa u tri standardna kultivara vinove loze Merlot, Cabernet Franc i Pinot Noir te u autohtonom kultivaru Tribidrag. Također, ispitan je učinak virusa GLRaV-3 u kombinaciji s različitim virusima koji se često mogu naći u poljskim inokulima (kao što su GLRaV-1, GLRaV-2, GVA, GPGV i drugi) na ispitivane kultivare. Mjereni indikatori oksidacijskog stresa su uključivali sadržaj ukupne količine topivih proteina, aktivnost enzima superoksid dismutaze (SOD), sadržaj vodikovog peroksida, malondialdehida i fotosintetskih pigmentata. Rezultati pokazuju različite stupnjeve osjetljivosti ispitivanih kultivara na infekciju testnim virusnim izolatima. U usporedbi s drugim kultivarima, utvrđeno je da je kultivar Tribidrag najosjetljiviji na infekciju sa sojevima virusa GLRaV-3 i s poljskim inokulima jer je u tom kultivaru uglavnom zabilježeno smanjenje količine topivih proteina te sadržaja klorofila b i karotenoida, indukcija aktivnosti SOD i akumulacija vodikovog peroksida. S obzirom na dobivene rezultate može se zaključiti da je infekcija testnim virusima izazvala nešto slabiji stres u kultivaru Merlot, dok kultivari Cabernet Franc i Pinot Noir bilježe minimalne promjene uslijed infekcije virusom GLRaV-3 i poljskim virusnim izolatima. U tim je kultivarima utvrđeno smanjenje količine fotosintetskih pigmenata kao dominantna promjena uslijed infekcije s virusom GLRaV-3. Saznanja dobivena u ovom radu mogu biti korištena kao smjernica za daljnja istraživanja utjecaja kombiniranih infekcija virusa na fiziologiju vinove loze.

Abstract (english)

The aim of this work was to investigate the effect of GLRaV-3 strains and their combinations on oxidative stress parameters using three standard red cultivars Merlot, Cabernet Franc and Pinot Noir as well as an indigenous Croatian wine grape variety Tribidrag. Also, the effect of GLRaV-3 in combination with various viruses that are common in field inoculums (such as GLRaV-1, GLRaV-2, GVA, GPGV and others) was examined in the grape cultivars. Total soluble proteins, superoxide dismutase (SOD) activity, hydrogen peroxide, malondialdehyde and photosynthetic pigments wre used as indicators of oxidative stress. The results show different degree of sensitivity of the tested cultivars to the infection caused by GLRaV-3 strains and field inoculums. In comparison with other cultivars, Tribidrag is the cultivar most susceptible to infection with GLRaV-3 and field inoculums as evidenced by generally decreased soluble protein content, chlorophyll b and carotenoids contents as well as enhanced SOD activity and hydrogen peroxide accumulation. Obtained dana suggest less negative effects in Merlot cultivar, while Cabernet Franc and Pinot Noir cultivars display minimum changes in response to the GLRaV-3 strains and field inoculums. In those cultivars, decrease of photosynthetic pigments was the dominat change in response to GLRaV-3 infection. Findings obtained in this study can be used as a a guideline for future studies of the impact of combined virus infections on grapevine.

• Keywords: biotički stres; Tribidrag; Merlot; Cabernet Franc; Pinot Noir; SOD; MDA
• Keywords (english): biotic stress; Tribidrag; Merlot; Cabernet Franc; Pinot Noir; SOD; MDA
• Language: croatian
• URN:NBN: urn:nbn:hr:217:181063
• Study programme: Title: Enviromental Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-05-13
• Created on: 2021-05-18 13:05:06