Populacijsko-genetička karakterizacija mikrosatelitnih biljega dalmatinskog buhača (Tanacetum cinerariifolium (Trevir.) Sch. Bip.)

master's thesis

• Title (english): Population-genetic characterization of microsatellite markers of Dalmatian pyrethrum (Tanacetum cinerariifolium (Trevir.) Sch. Bip.)
• Author: Sara Laura Šarančić
• Mentor: Zlatko Liber (mentor)
• Committee member: Zlatko Liber (predsjednik povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Committee member: Mirela Sertić Perić (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-07-06, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Dalmatinski buhač je endemična i zaštićena biljka istočne obale Jadranskog mora iz porodice glavočika (Asteraceae). Ekonomski najvažnija osobina ove vrste je sposobnost sinteze prirodnog insekticida piretrina. Postojanje podataka o genetičkoj raznolikosti i strukturi populacije nužno je za filogeografska istraživanja, programe oplemenjivanja bilja i odgovarajuću zaštitu neke vrste. Danas se za istraživanje genetičke raznolikosti i strukture populacija upotrebljavaju različiti DNA biljezi, a najčešće su upotrebljavani mikrosateliti. Cilj ovog diplomskog rada bio je utvrditi razinu informativnosti i primjenjivost 12 novorazvijenih mikrosatelitskih biljega. Nakon PCR amplifikacije mikrosatelitskih lokusa 96 jedinki iz pet prirodnih populacija i nakon statističke analize dobivenih podataka utvrđena je: dovoljna razina informativnosti za 11 mikrosatelitskih biljega, pozitivna korelacija između genetske raznolikosti i zemljopisnog položaja istraživanih populacija, najveća genetska raznolikost (HE), prosječni broj alela po lokusu (Nar) i najveći broj jedinstvenih alela (Npr) u tri najjužnije populacije, smanjena heterozigotnost zbog križanja u populaciji Lovćena, kao i širenje u populaciji Trebinje. Novorazvijeni mikrosatelitni biljezi predstavljat će važan alat za utvrđivanje podrijetla uzgajanih populacija, u budućim programima oplemenjivanja, te za generiranje podataka nužnih za učinkovito upravljanje i zaštitu ove biljne vrste.

Abstract (english)

Dalmatian pyrethrum (Tanacetum cinerariifolium /Trev. / Schultz Bip.) is an endemic and protected plant species from the east Adriatic coast that belong to the aster family (Asteraceae). The most important economic value of this species is its ability to synthesize the natural insecticide pyrethrin. The existence of data on genetic diversity and population structure is necessary for phylogeographic research, plant breeding programs and appropriate protection of a species. Today, various DNA markers are used to research genetic diversity and population structure. Among them, microsatellites are the most used. Determination of the level of the informativity and the applicability of 12 newly developed microsatellite markers was the aim of this graduation thesis. After PCR amplification of microsatellite loci of 96 individuals from five natural populations and after statistical analysis of obtained data, it was determined: sufficient level of the informativity for 11 microsatellite markers, positive correlation between genetic diversity and geographical position of the studied population, the largest genetic diversity (HE), the average number of alleles per locus (Nar) and the largest number of unique alleles (Npr) in three southernmost populations, reduced heterozygosity due to inbreeding in Lovćen population, as well as expansion in the Trebinje population. Newly developed microsatellite markers will be an important tool for determining the origin of cultivated populations, in future breeding programs, and for generating data necessary for the effective management and protection.

• Keywords: dalmatinski buhač; genetička raznolikost i struktura; mikrosatelitni biljezi; oplemenjivanje bilja; prirodni insekticid.
• Keywords (english): Dalmatian pyrethrum; genetic diversity and structure; microsatellite markers; plant breeding; natural insecticide.
• Language: croatian
• URN:NBN: urn:nbn:hr:217:644840
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2021-07-22 12:04:25