Kvaliteta otpadne vode smetlišta Piskornica prije i nakon pročišćavanja

master's thesis

• Title (english): Quality of Piskornica landfill wastewater prior to and following purification
• Author: Vid Baković
• Mentor: Sandra Radić Brkanac (mentor)
• Committee member: Sandra Radić Brkanac (predsjednik povjerenstva)
• Committee member: Renata Matoničkin Kepčija (član povjerenstva)
• Committee member: Nenad Buzjak (član povjerenstva)
• Committee member: Alan Moro (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2012-02-03, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

U ovom je radu procijenjena kvaliteta procjedne vode sakupljene sa starog odlagališta otpada Piškornica (Koprivnica) prije i nakon kemijskog ili elektrokemijskog pročišćavanja. Toksični i genotoksični učinci procjedne vode istraženi su na dva testna organizma, algi Chlorella vulgaris Beij. i vodenoj leći (Lemna minor L.), putem Lemna-, klorela- i komet-testa. Pokazatelji toksičnosti praćeni u vodenoj leći nakon tjedan dana izlaganja bili su sadržaj fotosintetskih pigmenata i malondialdehida (ukazuje na peroksidaciju membranskih lipida) te aktivnosti peroksidaza i katalaze. U toj su biljci uzorci procjedne vode izazvali oštećenje molekule DNA, smanjenje rasta i sadržaja pigmenata te povećanje sadržaja malondialdehida i aktivnosti antioksidacijskih enzima. Nakon tri i 14 dana izlaganja uzorcima voda, napravljena je morfološka i morfometrijska analiza stanica algi roda Chlorella. Uzorak procjedne vode uzrokovao je povećanje površine stanica i kloroplasta kao i dijametra stanica. Vrijednosti gotovo svih parametara mjerenih u algama i biljkama izloženim pročišćenim uzorcima procjedne vode bili su slični vrijednostima kontrolnih biljaka. Rezultati pokazuju da su obje metode pročišćavanja vrlo učinkovite u uklanjanju toksičnih tvari prisutnih u odlagališnom eluatu.

Abstract (english)

The present study evaluates the quality of leachate from an old sanitary landfill Piskornica, (Koprivnica, Croatia) prior to and following chemical and electrochemical treatments. Toxic and genotoxic effects of the leachate were observed on two test organisms, Lemna minor and Chlorella vulgaris Beij., by using Lemna, Chlorella and comet tests. Photosynthetic pigments, malondialdehyde (indicates damage to membrane lipids) and antioxidant enzymes catalase and peroxidase were evaluated as indicators of toxicity in duckweed following a week of exposure. When exposed to landfill leachate, growth rate and photosynthetic pigments content in duckweed decreased while enzyme activity, malondialdehyde content and DNA damage increased. The morphology and morphometric parameters were analyzed in algae of Chlorella genus after 3- and 14-day period of cultivation. Area of cells and chloroplasts and diameter of C.vulgaris cells increased in response to landfill leachate. Almost all parameters measured in plants and algae cultivated on treated leachate samples were similar to control, suggesting that both purification methods had been highly efficient in removal of toxic substances present in landfill leachate.

• Keywords: toksičnost; eluat; Lemna minor; Chlorella; biokemijski pokazatelji
• Keywords (english): toxicity; water treatment; Lemna minor; Chlorella; biochemical indicators
• Language: croatian
• URN:NBN: urn:nbn:hr:217:853099
• Study programme: Title: Enviromental Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-28 09:09:35