Učinak resveratrola na serumske markere oksidacijskog stresa u pacijenata podvrgnutih operaciji zamjene srčanog zaliska

master's thesis

• Title (english): The effects of resveratrol on markers of oxidative stress in serum of patients who underwent the heart valve replacement surgery
• Author: Kristina Mutabdžić
• Mentor: Dubravka Rašić (mentor)
• Mentor: Domagoj Đikić (mentor)
• Committee member: Domagoj Đikić (predsjednik povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-06-30, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Bolesti srca i krvnih žila glavni su uzrok smrti i bolničkog liječenja u Hrvatskoj. Srce može postati ranjivo od štetnih životnih navika kao što su pušenje, nezdrava prehrana ili prevelika razina stresa. Potencijalna uloga antioksidansa u prevenciji i liječenju kardiovaskularnih bolesti intenzivno se istražuje u posljednja četiri desetljeća. Resveratrol (RSV) se nalazi u kikirikiju i njegovim derivatima, pistacijama, šumskom voću, tamnoj čokoladi i grožđu te njegovim derivatima. Štiti kardiovaskularni sustav na više načina. Oksidacijski stres je definiran kao poremećaj u ravnoteži između antioksidansa i prooksidansa koji se očituje u povećanoj koncentraciji prooksidansa što potencijalno vodi oštećenju stanica. Kao biološki markeri oksidacijskog stresa u ovom istraživanju mjereni su malondialdehid (MDA) kao pokazatelj oksidacijskog oštećenja lipida i glutation (GSH) koji ima ulogu antioksidansa. Koncentracije MDA i GSH mjerene su u plazmi pacijenata uzorkovane u pet vremenskih točaka prije, tijekom i nakon operacije. Pacijenti su podijeljeni u dvije skupine koje su dobivale placebo (laktulozu) i RSV. Koncentracije MDA i GSH nisu se međusobno statistički značajno razlikovale u ove dvije skupine. Koncentracija GSH bila je statistički značajno veća u plazmi pacijenata uzorkovanoj po dolasku u jedinicu intenzivnog liječenja u odnosu na ostale točke uzorkovanja. Rezultati mjerenja oksidacijskog stresa ukazuju na malene promjene oksidacijskog stresa tijekom operacije zamjerne aortnog zaliska te protektivno djelovanje RSV-a, ali i laktuloze koja je korištena kao placebo. Potrebno je mjeriti veći broj čimbenika kako bismo dobili potpuniji uvid u utjecaj resveratrola na oksidacijski stres tijekom operacija.

Abstract (english)

Cardiovascular diseases are the main cause of death and hospital treatment in Croatia. The heart can become vulnerable to harmful life habits such as smoking, unhealthy diet or excessive levels of stress. The potential role of antioxidants in the prevention and treatment of cardiovascular disease has been intensively investigated in the last four decades. Resveratrol is found in peanuts and its derivatives, pistachios, forest fruits, dark chocolate and grapes and its derivatives. Resveratrol protects the cardiovascular system in several ways. Oxidative stress is a disturbance in the balance between antioxidants and prooxidants that manifests as an increased concentration of prooxidants which can lead to cell damage. As biological markers of oxidative stress in this study, malondialdehyde, as an indicator of lipid oxidative damage, and glutathione that plays the role of antioxidants, were measured. Blood samples were sampled for plasma five times before, during and after surgery. Patients were divided into two groups receiving placebo (lactulose) and resveratrol. There were no differences in plasma MDA and GSH concentrations between groups. GSH concentration was significantly increased in plasma taken after receiving patients in the intensive care unit. The results of oxidative stress indicate small changes in oxidative stress during aortic valve surgery and an antioxidative effect of RSV and lactulose. It is necessary to measure a number of factors to gain a complete insight into the effect of resveratrol on oxidative stress during operations.

• Keywords: glutation; malondialdehid; antioksidansi; kardiovaskularne bolesti
• Keywords (english): glutathione; malondialdehyde; antioxidant; cardiovascular disease
• Language: croatian
• URN:NBN: urn:nbn:hr:217:020680
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-06-30
• Created on: 2021-09-03 07:12:05