Karakterizacija karbapenemaza u enterobakterija

master's thesis

• Title (english): Characterization of carbapenemases in Enterobacteriacae
• Author: Marina Barišić
• Mentor: Branka Bedenić (mentor)
• Mentor: Mladen Krajačić (mentor)
• Committee member: Zdravko Dolenec (predsjednik povjerenstva)
• Committee member: Branka Bedenić (član povjerenstva)
• Committee member: Zora Popović (član povjerenstva)
• Committee member: Tajana Preočanin (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2012-12-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Karbapenemi su antibiotici stabilni prema većini β-laktamaza pa su često jedina terapijska opcija u liječenju infekcija uzrokovanih multirezistentnim enterobakterijama. Sve je češća pojava otpornosti enterobakterija na karbapeneme zbog proizvodnje KPC, VIM, NDM i OXA-48 β-laktamaza. Cilj rada bio je istražiti mehanizme rezistencije na karbapeneme. Izolati su bili prikupljani tokom 2011. i 2012. godine iz različitih bolničkih centara u Hrvatskoj. Testiranje osjetljivosti na antibiotike napravljeno je disk-difuzijskom i MIK metodom. Fenotipska detekcija ESBL enzima napravljena je metodom kombiniranih diskova po CLSI-u, detekcija metalo-β-laktamaza napravljena je pomoću imipenem/meropenem-EDTA disk-difuzijske metode a detekcija KPC enzima pomoću 3-aminofenilboronične kiseline. Detekcija gena rezistencije napravljena je pomoću lančane reakcije polimeraze početnicama specifičnim za ESBL, OXA-48, KPC, IMP, VIM, NDM i plazmidne AmpC -laktamaze. Svi sojevi koji su bili pozitivni na proizvodnju karbapenemaza posjedovali su gene blaESBL i blaampC što predstavlja ozbiljan problem u liječenju bolesti upravo zbog multirezistencije koju pokazuju.

Abstract (english)

Carbapenems are antibiotics that are frequently used to treat infections with multidrug-resistant Enterobacteriaceae because they are not inactivated by most β-lactamases. However, β-lactamase mediated resistance to carbapenems has been reported in Enterobacteriaceae, mostly due to the expression of KPC, VIM, NDM or OXA-48 β-lactamase. The purpose of this research was to clarify the resistance mechanisms to carbapenem antibiotics. The isolates were gathered in several hospital institutions in Croatia. The antimicrobial susceptibility to a wide range of antibiotics was determined by disk-diffusion and MIK test. Detection of ESBLs was done by CLSI confirmatory test, detection of metallo-β-lactamases was done by imipenem/meropenem-EDTA test and detection of KPC enzime was done by 3-aminophenylboronic acid test. The presence of genes encoding β-lactamases was determined by PCR using primers for ESBL, OXA-48, KPC, IMP, VIM, NDM i plasmid-mediated AmpC beta-lactamases. The fact that most of the strain possessed not only blacarb genes but also blaESBL and plasmid-mediated blaampC genes limits therapeutic options.

• Keywords: AmpC; ESBL; KPC; metalo-β-laktamaze; multirezistencija
• Keywords (english): AmpC; ESBLs; KPC; metallo-β-lactamases; multidrug-resistance
• Language: croatian
• URN:NBN: urn:nbn:hr:217:332055
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-28 09:28:43