Biomonitoring onečišćenja Vranskog jezera kaveznim izlaganjem školjkaša Unio pictorum (Linnaeus, 1758)

master's thesis

• Title (english): Biomonitoring of Vrana lake by cage exposure of mussel Unio pictorum (Linnaeus 1758)
• Author: Luka Basrek
• Mentor: Goran Klobučar (mentor)
• Committee member: Goran Klobučar (predsjednik povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Mirjana Pavlica (član povjerenstva)
• Committee member: Ivančica Ternjej (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2012-07-11, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Onečišćenje vodenih ekosustava može dovesti do promjena na molekularnoj i staničnoj razini organizama koji ih nastanjuju prije nego se oštećenje manifestira na razini fizioloških procesa cijelog organizma. U ovom radu procijenjeno je oksidativno oštećenje makromolekula te oštećenje DNA u test organizmu (slikarska lisanka, Unio pictorum L.) kavezno izloženom na nekoliko postaja na Vranskom jezeru (Kanal Kotarka, Lateralni kanal, Sredina jezera, Prosika). Školjkaši su prikupljeni na referentnoj postaji Draganići te izloženi u proljeće i jesen 2011 godine. Biomarkeri genotoksičnosti (komet- i mikronukleus-test) te biokemijski pokazatelji stresa (sadržaj topivih proteina, sadržaj malondialdehida i sadržaj karbonilnih grupa) analizirani su na izloženim školjkašima te školjkašima sa referentne postaje. Oštećenje DNA te oksidacijska oštećenja makromolekula zabilježena su na postajama Kanal Kotarka i Lateralni kanal u odnosu na referentnu postaju što ukazuje na prisutnost genotoksikanata kao i tvari koje uzrokuju oksidacijska oštećenja u Vranskom jezeru.

Abstract (english)

Pollution of aquatic ecosystems can cause changes on molecular and cellular level in organisms inhabiting them They usually occur before toxic action becomes apparent in physiology of the entire organism. In this study, oxydative damage of macromolecules and DNA damage were examined using caged painter's mussels (Unio pictorum L.) as test organism. Mussels were cage exposed at several locations on Vrana lake (Channel Kotarka, Lateral channel, Lake centre, Prosika). Mussels were collected at the reference site (Draganići), and exposed during spring and autumn 2011. Biomarkers of genotoxicity (Comet assay and micronucleus-test) and biochemical indicators of stress (total proteins, malondialdehyde, carbonyl groups) were analysed on mussels exposed in Vrana Lake and the ones from the reference site. DNA damage and oxidative damage of macromolecules were recorded on Channel Kotarka and Lateral channel which indicates presence of genotoxicants as well asagents which cause oxidative damage in Vrana Lake.

• Keywords: Unio pictorum; hemociti; komet-test; mikronukleus-test; malondialdehid; karbonili; oksidativno oštećenje; okolišno onečišćenje
• Keywords (english): Unio pictorum; haemocytes; comet-test; micronucleus-test; malondialdehyde; carbonyl groups; oxydative damage; environmental contamination
• Language: croatian
• URN:NBN: urn:nbn:hr:217:716330
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-28 09:35:16