Pokazatelji toksičnosti nano čestica srebra u listovima duhana (Nicotiana tabacum L.)

master's thesis

• Title (english): Toxicity of silver nanoparticles in tobacco (Nicotiana tabacum L.)
• Author: Dijana Beneta
• Mentor: Sandra Radić Brkanac (mentor)
• Committee member: Sandra Radić Brkanac (predsjednik povjerenstva)
• Committee member: Ivančica Ternjej (član povjerenstva)
• Committee member: Vesna Benković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2012-02-09, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Nano čestice srebra se zbog svog antimikrobnog svojstva sve više koriste u proizvodima široke potrošnje (dezodoransima, parfemima, odjeći i obući, zavojima te u sredstvima za pranje i čišćenje) kao i u raznim industrijskim granama. Dosad je zabilježen fito- , cito- i genotoksični učinak nano čestica srebra na pojedine biljne i animalne organizme. U ovom je radu istražen toksični učinak nano čestica srebra (u rasponu koncentracija od 5 do 200 mg L-1) na listove duhana (Nicotiana tabacum L.) koji su uzorkovani nakon tjedan dana izlaganja. Određen je sadržaj klorofila i karotenoida te su izmjereni pojedini pokazatelji oksidacijskog stresa i aktivnosti antioksidacijskih enzima. Nano čestice srebra značajno su povećale sadržaj fotosintetskih pigmenata pri višim koncentracijama nano srebra. Zabilježen je i porast prolina te smanjenje sadržaja proteina. Nano čestice srebra su pri višim koncentracijama povećale oksidacijsko oštećenje lipida. Zamijećena je povećana aktivnost enzima superoksid dismutaze na tretmanima s najvišim koncentracijama nano srebra dok su porast sadržaja glutation reduktaze uzrokovale niže koncentracije nano srebra. Aktivnost askorbat peroksidaze, enzima koji razgrađuje vodikov peroksid, bila je povećana na svim tretmanima. Rezultati ovog istraživanja ukazuju da nano čestice srebra izazivaju oksidacijski stres u izdanku duhana, ali i na učinkovitost antioksidacijske obrane duhana pri koncentracijama tog toksičnog metala upotrebljenim u ovom radu.

Abstract (english)

Nanoparticles of silver, due to its antimicrobial properties, are increasingly used in consumer products (deodorants, perfumes, clothes and shoes, bandages and in cleaning solutions and sprays) as well as in various industries. So far, phyto-, cyto-and genotoxic effects of silver nanoparticles to the individual plant and animal organisms were observed. This paper investigated the toxic effect of silver nano particles (in the concentration range of 5-200 mg L-1) in tobacco (Nicotiana tabacum L.). Plants were harvested and analyzed after 7 days. Chlorophylls and carotenoid contents, certain indicators of oxidative stress and antioxidant enzyme activities were measured. Silver nanoparticles, at higher concentrations, significantly increased the contents of photosynthetic pigments. Also, an increase of proline and decrease in protein content was noticed. At higher concentrations, silver nanoparticles increased oxidative damage to membrane lipids. An increased activity of the enzyme superoxide dismutase has been noticed under higher nano silver treatments, while the induction of glutathione reductase was caused by lower nano silver concentrations. The activity of ascorbate peroxidase was increased at all treatments. The results of the study indicate that silver nano particles cause oxidative stress in N. tabacum leaves, but they also show the efficiency of antioxidative defense system of the tobacco within the range of nano silver concentrations used in the work.

• Keywords: nano čestice srebra; Nicotiana tabacum; toksičnost; oksidacijski stres; antioksidacijski enzimi
• Keywords (english): nanoparticles; silver; Nicotiana tabacum; toxicity; oxidative stress; antioxidative enzymes
• Language: croatian
• URN:NBN: urn:nbn:hr:217:537539
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-28 09:37:23