Promjena raznolikosti tipova vegetativne kompatibilnosti i prevalencije hipovirulentnih izolata gljive Cryphonectria parasitica u populacijama na području Ozlja i Kašta

undergraduate thesis

• Title (english): Change in vegetative compatibility diversity and hypovirulence prevalence in Cryphonectria parasitica isolates from Ozalj and Kašt area populations
• Author: Lovro Ogresta
• Mentor: Marin Ježić (mentor)
• Committee member: Marin Ježić (predsjednik povjerenstva)
• Committee member: Marija Gligora Udovič (član povjerenstva)
• Committee member: Ivan Čanjevac (član povjerenstva)
• Committee member: Hana Fajković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-09-21, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Cryphonectria parasitica je parazitska gljiva mješinarka, uzročnik bolesti raka kore kestena, koja može biti pogubna za svog domaćina. Infekcijom gljive virusom Cryphonectria hypovirus 1 (CHV1) dolazi do smanjenja njene virulencije (usporavanje rasta i slabija sporulacija micelija) te cijeljenja rakova. CHV1 se lako prenosi između micelija gljiva istog tipa vegetativne kompatibilnosti (vc), koji je definiran s 6 dialelnih lokusa vegetativne nekompatibilnosti (vic). Cilj ovog istraživanja bio je istražiti promjene u raznolikosti vc tipova i prevalenciji CHV1 u populacijama C. parasitica na području Ozlja i Kašta u 2014. i 2019. godini. Rezultati dobiveni RT-PCR testom i metodom koinokulacije uzoraka i standardnih testera pokazali su da se struktura ni jedne populacije nije značajno promijenila tijekom pet godina. Zbilježena su dva nova vc tipa u populaciji Kašta i osam u populaciji Ozlja, od kojih su tri u potpunosti nova na području Republike Hrvatske. Prevalencija hipovirulencije i distribucija vc tipova među hipovirulentnim izolatima nisu se značajno promijenile ni u jednoj populaciji. Na temelju ovih rezultata može se zaključiti da prema zastupljenosti vc tipova i raspodjeli CHV1 po raznim vc tipovima, obje populacije za sada stagniraju.

Abstract (english)

Cryphonectria parasitica is a parasitic fungus that causes chestnut bligh, which can be fatal for its host. Infection of the fungus with Cryphonectria hypovirus 1 (CHV1) leads to reduced virulence (i.e. slower growth and decreased sporulation of the mycelium) and healing of cankers. The virus is transmitted among individuals of the same vegetative compatibility (vc) type, controlled by 6 diallelic vegetative incompatibility (vic) loci. The aim of this study was to investigate changes in the diversity of vegetative compatibility and the prevalence of CHV1 in populations of C. parasitica in the area of Ozalj and Kašt in 2014 and 2019. The results obtained by RT-PCR test and pairing of the samples with standard testing strains showed that the structure of neither population changed significantly over the last five years. Two new genotypes were recorded in the population of Kašt and eight in the population of Ozalj, three of which have not been observed so far on the territory of the Republic of Croatia. The prevalence of hypovriulence and the distribution of vc types among hypovirulent isolates did not change significantly in either population. Based on these results, it can be concluded that two populations are in the state of stagnation in regard to vc type ratios and hipovriulence prevalence.

• Keywords: biološka kontrola; rak kore kestena; RNA virus
• Keywords (english): biolocontol; chestnut blight; RNA virus
• Language: croatian
• URN:NBN: urn:nbn:hr:217:562028
• Study programme: Title: Environmental Sciences; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) struke znanosti o okolišu (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-09-21
• Created on: 2021-09-29 11:09:40