Genska struktura i raznolikost velike pliske, Alburnus sarmaticus Freyhof & Kottelat, 2007 u Kupi i Mrežnici

master's thesis

• Title (english): Genetic structure and diversity of Pontian shemaya, Alburnus sarmaticus Freyhof & Kottelat, 2007 in the Kupa and Mrežnica Rivers
• Author: Ana Razum
• Mentor: Ivana Buj (mentor)
• Committee member: Ivana Buj (predsjednik povjerenstva)
• Committee member: Iva Juranović Cindrić (član povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Glavni cilj ovog diplomskog rada bio je utvrditi i analizirati raznolikost i gensku strukturu populacija vrste Alburnus sarmaticus (velika pliska) u Hrvatskoj. Ova vrsta u Europi obitava samo u crnomorskom slijevu, i to u nizvodnim dijelovima rijeke Dunav, te u Dnjeparu i Južnom Bugu na području Rumunjske, Ukrajine i Bugarske, dok se u Hrvatskoj može naći potpuno izolirana populacija ove vrste u rijekama Savi, Kupi i Mrežnici. Uzorci su prikupljeni na rijekama Mrežnica i Kupa. Analiza podataka je uključivala izolaciju ukupne genomske DNA, umnažanje odabranih genskih markera lančanom reakcijom polimerazom, sravnjivanje sekvenci, što sam provela pomoću programa BioEdit, prilikom čega su uočene i pojedine mutacije među sekvencama. Također sam izradila izradila filogenetska stabla pomoću programa PAUP, prilikom čega sam koristila metode najveće parsimonije i najveće vjerojatnosti. Utvrdila sam da sekvence velike pliske pripadaju monofiletskoj liniji. Zatim sam izradila filogenetsku mrežu dobivenih haplotipova korištenjem programa Network. Za utvrđivanje stupnja intrapopulacijske genske raznolikosti koristila sam program DnaSP. Intraspecijsku i interpopulacijsku strukturu velike pliske u Hrvatskoj detaljno sam analizirala korištenjem programa MIGRATE. Filogenetska stabla pokazuju kako vrsta A. sarmaticus pripada staroj monofiletskoj liniji. Rezultati su također pokazali visoku gensku raznolikost, što je vrlo pozitivan pokazatelj za budući povoljan razvitak ove vrste.

Abstract (english)

The main goal of this thesis was to determine and analyze the diversity and genetic structure of populations of Alburnus sarmaticus (Pontian shemaya) in Croatia. In Europe, this species lives only in the Black Sea basin, in the downstream parts of the Danube, and in the Dnieper and Southern Bug in Romania, Ukraine and Bulgaria, while in Croatia a completely isolated population of this species can be found in the Sava, Kupa and Mrežnica Rivers. Samples were collected from the Mrežnica and Kupa Rivers. Data analysis includes isolation of total genomic DNA, amplification of selected gene markers by polymerase chain reaction, sequence alignment, which I performed using the BioEdit program, during which individual mutations between sequences were observed. I also made phylogenetic trees using the PAUP program, using the methods of the highest parsimony and the highest probability. I determined that the sequences of the Pontian shemaya belong to the monophyletic line. I then constructed a phylogenetic network of the obtained haplotypes using the Network program. I used the DnaSP program to determine the degree of intrapopulation gene diversity. I analyzed the intraspecific and interpopulation structure of the Pontian shemaya in Croatia in detail using the MIGRATE program. Phylogenetic trees show that the species A. sarmaticus belongs to the old monophyletic line. The results also showed high genetic diversity, which is a very positive indicator for the future favorable development of these species.

• Keywords: velika pliska; genska raznolikost; genska struktura; filogenijA
• Keywords (english): Pontian shemaya; genetic diversity; genetic structure; phylogeny
• Language: croatian
• URN:NBN: urn:nbn:hr:217:979113
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-09-27
• Created on: 2021-10-06 09:50:06