Mikrobiološka analiza i utvrđivanje najčešćih uzročnika kontaminacije u Banci tkiva i stanica

master's thesis

• Title (english): Microbiological analysis and identification of the most common causes of contamination in The Tissue and Cell Bank
• Author: Lucija Pecko
• Mentor: Marija Zekušić (mentor)
• Mentor: Inga Urlić (sumentor)
• Committee member: Inga Urlić (predsjednik povjerenstva)
• Committee member: Romana Gračan (član povjerenstva)
• Committee member: Ivan Radosavljević (član povjerenstva)
• Committee member: Željka Vidaković-Cifrek (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-09-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Banka tkiva i stanica (BTS) u Zavodu za transfuzijsku i regenerativnu medicinu Kliničkog bolničkog centra Sestre milosrdnice je organizirana klinička jedinica u kojoj se obavljaju djelatnosti prikupljanja, uzimanja, obrade, očuvanja, pohrane i raspodjele ljudskih tkiva i stanica namijenjenih za kliničku primjenu. Unutar BTS izgrađen je Laboratorij za tkivno inženjerstvo u skladu s dobrom proizvođačkom praksom (engl. Good Manufacturing Practice, GMP) i prema tehnologiji čistih prostorija. U laboratoriju gdje se radi sa stanicama/tkivima ljudskog porijekla ključno je kontinuirano uzorkovati zrak, kontaktne površine, laboratorijsku opremu, odjeću djelatnika, prste, vodu iz kritične laboratorijske opreme kao i hranjivi stanični medij, alikvote i bioptate tkivnih presadaka. Cilj je, kontinuiranim uzorkovanjem, utvrditi vrstu i broj kolonija najučestalijih mikroorganizama te utvrditi njihov značaj za sigurnost cijelog procesa aseptične proizvodnje tkiva i stanica. Laboratorijski djelatnici predstavljaju glavni izvor kontaminacije što potvrđuje činjenica da je u svim klasama čistog prostora najučestalije bakterije iz rodova Staphylococcus (koagulaza negativni stafilokoki, CoNS), Micrococcus i Bacillus. Mikrobiološka analiza uzoraka provodi se na Odjelu za mikrobiologiju pri Hrvatskom zavodu za transfuzijsku medicinu s ciljem detekcije prisutnosti aerobnih i anaerobnih bakterija te kvasaca i plijesni. Dozvoljen broj kolonija za čiste prostorije definiran je prema GMP standardu te ne smije biti prekoračen.

Abstract (english)

The Tissue and Cell Bank (TCB) at the Department of Transfusion and Regenerative Medicine of the Sestre milosrdnice University Hospital Center is an organized clinical unit in which the activities of collecting, taking, processing, preserving, storing and distributing human tissues and cells intended for clinical use are performed. Within TCB, a Tissue Engineering Laboratory was built in accordance with Good Manufacturing Practice (GMP) and clean room technology. In a laboratory working with cells / tissues of human origin, it is crucial to control the level of cleanliness of air, contact surfaces, laboratory equipment, staff clothing, water from critical laboratory equipment as well as nutrient cell medium, aliquots and tissue grafts. The aim is to determine the type and number of colonies of the most common microorganisms by continuous sampling and to determine their importance for the safety of the entire process of aseptic tissue and cell production. Laboratory workers are the main source of contamination, which is confirmed by the fact that in all classes of clean space, the most common bacteria are Staphylococcus (coagulase-negative staphylococcus, CoNS), Micrococcus and Bacillus. Microbiological analysis of samples is performed at the Department of Microbiology at the Croatian Institute of Transfusion Medicine with the aim of detecting the presence of aerobic and anaerobic bacteria and yeasts and molds. The permitted number of colonies for clean rooms is defined according to the GMP standard and must not be exceeded.

• Keywords: čiste prostorije; praćenje okoliša; mikroorganizmi; tkivo; stanica
• Keywords (english): clean rooms; environmental monitoring; microorganisms; tissue; cell
• Language: croatian
• URN:NBN: urn:nbn:hr:217:743688
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-09-28
• Created on: 2021-10-08 10:25:22