Utjecaj nedostatka Tff3 proteina na ekspresiju jetrenih gena uključenih u apoptozu i metabolizam lipida i glukoze u mišjem modelu starenja

master's thesis

• Title (english): Effect of Tff3 protein deficiency on expression of apoptotic, lipid and glucose metabolisam relevant genes in aging mouse model
• Author: Jelena Štambuk
• Mentor: Mirela Baus Lončar (mentor)
• Mentor: Sofia Ana Blažević (sumentor)
• Committee member: Sofia Ana Blažević (predsjednik povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Sven Jelaska (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-09-24, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Tff3 protein pripada skupini Tff proteina koje karakterizira domena s tri petlje nalik trolisnoj djetelini po kojoj su dobili ime. Eksprimiran je u raznim tkivima te ima višestruku funkciju, ovisno o mjestu ekspresije i uvjetima u stanici. Dosadašnja istraživanja su pokazala da Tff3 ima antiapoptotsko djelovanje te da utječe na metabolizam glukoze i lipida u jetri, no točni mehanizmi njegova djelovanja nisu još do kraja proučeni. Apoptoza i metabolizam jetre se mijenjaju starenjem zbog nakupljanja promjena u organizmu. Starenjem se u jetri povećava učestalost apoptoze, dolazi do nakupljanja lipida i smanjene β-oksidacije i ketogeneze što često rezultira razvojem bolesti. Cilj ovog istraživanja bio je proučiti utjecaj nedostatka Tff3 na ekspresiju apoptotskih gena Bax, Smac, Cyc te gena vezanih uz metabolizam jetre Hmgcs2, Cpt1a, Gk u jetri miševa starih dvije godine. Ekspresiju gena pratili smo metodom kvantitativne lančane reakcije polimerazom, koju smo prethodno optimizirali podešavajući uvjete reakcije i isprobavanjem različitih početnica za relevantne gene. Usporedbom ekspresije gena miševa s nedostatkom proteina sa divljim tipom i statističkom analizom ustanovili smo da nije došlo do statistički značajne promjene, odnosno da nedostatak Tff3 proteina nije izazvao promjenu u ekspresiji ovih jetrenih gena u starim miševima.

Abstract (english)

The Tff3 protein belongs to a group of trefoil factor family (Tff) peptides that are characterized by the three-loop clover-like domain. It is expressed in multiple tissues and has various functions including effects on apoptosis. The exact function depends on the site of expression and conditions in the cell. Tff3 affects glucose and lipid metabolism in the liver. The exact mechanisms of its action are still a mystery. Apoptosis and liver metabolism change during aging due to progressive changes in the organism. Pathological changes during aging are caused by increased frequency of apoptosis and lipid accumulation, decreases β-oxidation and ketogenesis. This research aimed to study the effect of Tff3 deficiency on the expression of apoptotic genes Bax, Smac, Cyc, and genes related to liver metabolism Hmgcs2, Cpt1a, Gk in the liver of two-year-old mice. We used the quantitative polymerase chain reaction method, but first, we optimized the method by adjusting the conditions and testing different primers for relevant genes. Comparison of gene expression of protein-deficient mice with wild-type and statistical analysis revealed no statistically significant changes. Tff3 protein deficiency did not cause a change in the expression of these liver genes in old mice.

• Keywords: Tff3; jetra; starenje; apoptoza; metabolizam
• Keywords (english): Tff3; liver; aging; apoptosis; metabolizam
• Language: croatian
• URN:NBN: urn:nbn:hr:217:525018
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2022-09-24
• Created on: 2021-10-08 10:35:22