Genska struktura i raznolikost mladice, Hucho hucho (Linnaeus, 1758) u Hrvatskoj

master's thesis

• Title (english): Genetic structure and diversity of Danube salmon, Hucho hucho (Linnaeus, 1758) in Croatia
• Author: Sara Perčić
• Mentor: Ivana Buj (mentor)
• Committee member: Ivana Buj (predsjednik povjerenstva)
• Committee member: Mirela Sertić Perić (član povjerenstva)
• Committee member: Iva Juranović Cindrić (član povjerenstva)
• Committee member: Nenad Judaš (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2021-09-27, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Mladica je autohtona vrsta i endem dunavskog slijeva te se nalazi na Crvenom popisu IUCN-a kao ugrožena vrsta. Najveći razlog njezine ugroženosti je fragmentacija staništa uzrokovana izgradnjom brana i pregrada na vodotocima što je uzrokovalo smanjenje i nestajanje populacija. Iako se brojnost mladice od kraja 19. stoljeća smanjila za dvije trećine, stabilne populacije mladica u Hrvatskoj zabilježene su na granici sa Slovenijom u Kupi i na granici s Bosnom i Hercegovinom u Uni. Cilj ovog istraživanja bio je opisati gensku strukturu mladice u Hrvatskoj, utvrditi stupanj intraspecijske i intrapopulacijske genske raznolikosti, procijeniti zavičajnost pojedinih populacija mladica i njihovu različitost u odnosu na ostale europske populacije te istražiti evolucijsku povijest mladice u Hrvatskoj. Provedena je filogenetska rekonstrukcija temeljena na sekvencama za gen citokrom b (cyt b) i rodopsin (rhod) metodama najveće parsimonije (MP) i najveće vjerojatnosti (ML). Dobivena filogenetska stabla i mreža utvrdili su postojanje evolucijski odvojene linije mladice. Utvrđeno je jasno odvajanje roda Hucho unutar Salmonidae. Utvrđeno je da je genska raznolikost mladice na području dunavskog slijeva vrlo niska te da su migracije između populacija minimalne ili ne postoje. Niska genska raznolikost rezultat je prelova te izgradnje pregrada u vodotocima što uzrokuje fragmentaciju staništa.

Abstract (english)

Danube salmon is an indigenous species, endemic to the Danube basin and it is on the IUCN Red List as an endangered species. The biggest reason for its endangerment is habitat fragmentation caused by the construction of dams and barriers on watercourses, which has caused the reduction and disappearance of populations. Although population size has decreased by two thirds since the end of the 19th century, stable populations of Danube salmon in Croatia have been recorded on the border with Slovenia in Kupa and on the border with Bosnia and Herzegovina in Una. The aim of this study was to describe the genetic structure of Danube salmon in Croatia, to determine the degree of intraspecific and intrapopulation genetic diversity, to assess the origin of individual populations of Danube salmon and their diversity in relation to other European populations and to investigate the evolutionary history of Danube salmon in Croatia. Phylogenetic reconstruction based on sequences for the cytochrome b (cyt b) and rhodopsin (rhod) gene was performed using the maximum parsimony (MP) method and maximum probability (ML) method. The resulting phylogenetic trees and network determined the existence of an evolutionarily separate lineage for Danube salmon. A clear separation of the genus Hucho within Salmonidae was determined. It was found that the genetic diversity of Danube salmon in the Danube basin is very low and that migrations between populations are minimal or non-existent. Low genetic diversity is the result of overfishing and the construction of dams in watercourses, which causes habitat fragmentation.

• Keywords: mladica; dunavski slijev; filogenija; citokrom b; rodopsin
• Keywords (english): Danube salmon; Danube drain; phylogeny; cytochrome b; rhodopsin
• Language: croatian
• URN:NBN: urn:nbn:hr:217:798556
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2023-09-27
• Created on: 2021-10-08 11:21:09