The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin

Trupinić, Monika; Kokanović, Barbara; Ponjavić, Ivana; Barišić, Ivan; Šegvić, Siniša; Ivec, Arian; Tolić, Iva M.

doi:10.1016/j.cub.2022.04.035

prikaz prve stranice dokumenta The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin

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The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin

Current Biology, 32 (2022), 11; 2480-2493.e6. https://doi.org/10.1016/j.cub.2022.04.035

Trupinić, Monika; Kokanović, Barbara; Ponjavić, Ivana; Barišić, Ivan; Šegvić, Siniša; Ivec, Arian; Tolić, Iva M.

Citirajte ovaj rad

APA 6th Edition

Trupinić, M., Kokanović, B., Ponjavić, I., Barišić, I., Šegvić, S., Ivec, A. i Tolić, I. M. (2022). The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin. Current Biology, 32. (11), 2480-2493.e6. doi: 10.1016/j.cub.2022.04.035

MLA 8th Edition

Trupinić, Monika, et al. "The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin." Current Biology, vol. 32, br. 11, 2022, str. 2480-2493.e6. https://doi.org/10.1016/j.cub.2022.04.035

Chicago 17th Edition

Trupinić, Monika, Barbara Kokanović, Ivana Ponjavić, Ivan Barišić, Siniša Šegvić, Arian Ivec i Iva M. Tolić. "The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin." Current Biology 32, br. 11 (2022): 2480-2493.e6. https://doi.org/10.1016/j.cub.2022.04.035

Harvard

Trupinić, M., et al. (2022) 'The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin', Current Biology, 32(11), str. 2480-2493.e6. doi: 10.1016/j.cub.2022.04.035

Vancouver

Trupinić M, Kokanović B, Ponjavić I, Barišić I, Šegvić S, Ivec A, i sur.. The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin. Current Biology [Internet]. 06.2022. [pristupljeno 13.11.2024.];32(11):2480-2493.e6. doi: 10.1016/j.cub.2022.04.035

IEEE

M. Trupinić, et al., "The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin", Current Biology, vol. 32, br. 11, str. 2480-2493.e6, Lipanj 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:217:003399. [Citirano: 13.11.2024.]

Za citiranje koristite ovu mrežnu adresu: https://urn.nsk.hr/urn:nbn:hr:217:003399

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Naslov (engleski)	The chirality of the mitotic spindle provides a mechanical response to forces and depends on microtubule motors and augmin
Autor	Monika Trupinić
Autor	Barbara Kokanović
Autor	Ivana Ponjavić
Autor	Ivan Barišić
Autor	Siniša Šegvić
Autor	Arian Ivec
Autor	Iva M. Tolić
Autorova ustanova	Sveučilište u Zagrebu Prirodoslovno-matematički fakultet (Fizički odsjek)
Znanstveno / umjetničko područje, polje i grana	PRIRODNE ZNANOSTI Fizika
Sažetak (engleski)	Forces produced by motor proteins and microtubule dynamics within the mitotic spindle are crucial for proper chromosome segregation. In addition to linear forces, rotational forces or torques are present in the spindle, which are reflected in the left-handed twisted shapes of microtubule bundles that make the spindle chiral. However, the biological role and molecular origins of spindle chirality are unknown. By developing methods for measuring the spindle twist, we show that spindles are most chiral near the metaphase-to-anaphase transition. To assess the role of chirality in spindle mechanics, we compressed the spindles along their axis. This resulted in a stronger left-handed twist, suggesting that the twisted shape allows for a mechanical response to forces. Inhibition or depletion of motor proteins that perform chiral stepping, Eg5/kinesin-5, Kif18A/kinesin-8, MKLP1/kinesin-6, and dynein, decreased the left-handed twist or led to right- handed twist, implying that these motors regulate the twist by rotating microtubules within their antiparallel overlaps or at the spindle pole. A right-handed twist was also observed after the depletion of the microtubule nucleator augmin, indicating its contribution to the twist through the nucleation of antiparallel bridging microtubules. The uncovered switch from left- handed to right-handed twist reveals the existence of competing mechanisms that promote twisting in opposite directions. As round spindles are more twisted than the elongated ones are, we infer that bending and twisting moments are generated by similar molecular mechanisms and propose a physiological role for spindle chirality in allowing the spindle to absorb mechanical load.
Ključne riječi (engleski)
Jezik	engleski
Vrsta publikacije	Znanstveni rad - Izvorni znanstveni rad
Status objave	Objavljen
Vrsta recenzije	Recenziran - međunarodna recenzija
Verzija publikacije	Objavljena verzija rada (izdavačev PDF)
Naslov časopisa	Current Biology
Brojčani podaci	vol. 32, br. 11, str. 2480-2493.e6
p-ISSN	0960-9822
DOI	https://doi.org/10.1016/j.cub.2022.04.035
URN:NBN	urn:nbn:hr:217:003399
Datum objave publikacije	2022-06
Projekt	Šifra: PZS-2019-02-7653 Naziv (hrvatski): Mehanizmi nastajanja snopova mikrotubula potrebni za sazrijevanje diobenog vretena Naziv (engleski): Mechanisms of microtubule bundle formation required for spindle maturation Kratica: SpindleForm Voditelj: Iva Marija Tolić Pravna nadležnost: Hrvatska Financijer: Hrvatska zaklada za znanost Linija financiranja: Programme Research Cooperability
Projekt	Šifra: IP-2019-04-5967 Naziv (hrvatski): Ravnoteža sila i momenta sila u diobenom vretenu Naziv (engleski): Balance of forces and torques in the mitotic spindle Kratica: SpindleForceBalance Voditelj: Nenad Pavin Pravna nadležnost: Hrvatska Financijer: Hrvatska zaklada za znanost Linija financiranja: Research Projects
Projekt	Šifra: KK.01.1.1.04.0057 Naziv (hrvatski): Inovativni protokoli mikroskopije za interdisciplinarna istraživanja u biomedicini Naziv (engleski): Inovative microscopy protocols for interdisciplinary research in biomedicine Kratica: IPSted Voditelj: Iva Marija Tolić Pravna nadležnost: eu Financijer: Europska komisija Linija financiranja: European Regional Development Fund
Projekt	Šifra: 855158 Naziv (hrvatski): Molekularno podrijetlo aneuploidija u zdravim i bolesnim ljudskim tkivima Naziv (engleski): Molecular origins of aneuploidies in healthy and diseased human tissues Kratica: ANEUPLOIDY Voditelj: Iva Marija Tolić Pravna nadležnost: eu Financijer: Europska komisija
URL dokumenta	https://linkinghub.elsevier.com/retrieve/pii/S0960982222006042
Vrsta resursa	Tekst
Prava pristupa	Otvoreni pristup
Uvjeti korištenja
Javna napomena	© 2022 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Received: September 23, 2021. Received in revised form: February 17, 2022. Accepted: April 13, 2022. Published: May 9, 2022.
Datum i vrijeme pohrane	2024-08-22 19:52:46