Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features

Velle, Katrina B.; Kennard, Andrew S.; Trupinić, Monika; Ivec, Arian; Swafford, Andrew J.M.; Nolton, Emily; Rice, Luke M.; Tolić, Iva M.; Fritz-Laylin, Lillian K.; Wadsworth, Patricia

doi:10.1016/j.cub.2022.01.034

prikaz prve stranice dokumenta Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features

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Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features

Current Biology, 32 (2022), 6; 1247-1261.e6. https://doi.org/10.1016/j.cub.2022.01.034

Velle, Katrina B.; Kennard, Andrew S.; Trupinić, Monika; Ivec, Arian; Swafford, Andrew J.M.; Nolton, Emily; Rice, Luke M.; Tolić, Iva M.; Fritz-Laylin, Lillian K.; Wadsworth, Patricia

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APA 6th Edition

Velle, K. B., Kennard, A. S., Trupinić, M., Ivec, A., Swafford, A. J., Nolton, E. ... Wadsworth, P. (2022). Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features. Current Biology, 32. (6), 1247-1261.e6. doi: 10.1016/j.cub.2022.01.034

MLA 8th Edition

Velle, Katrina B., et al. "Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features." Current Biology, vol. 32, br. 6, 2022, str. 1247-1261.e6. https://doi.org/10.1016/j.cub.2022.01.034

Chicago 17th Edition

Velle, Katrina B., Andrew S. Kennard, Monika Trupinić, Arian Ivec, Andrew J.M. Swafford, Emily Nolton, Luke M. Rice, Iva M. Tolić, Lillian K. Fritz Laylin i Patricia Wadsworth. "Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features." Current Biology 32, br. 6 (2022): 1247-1261.e6. https://doi.org/10.1016/j.cub.2022.01.034

Harvard

Velle, K. B., et al. (2022) 'Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features', Current Biology, 32(6), str. 1247-1261.e6. doi: 10.1016/j.cub.2022.01.034

Vancouver

Velle KB, Kennard AS, Trupinić M, Ivec A, Swafford AJ, Nolton E, i sur.. Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features. Current Biology [Internet]. 03.2022. [pristupljeno 22.01.2025.];32(6):1247-1261.e6. doi: 10.1016/j.cub.2022.01.034

IEEE

K. B. Velle, et al., "Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features", Current Biology, vol. 32, br. 6, str. 1247-1261.e6, Ožujak 2022. [Online]. Dostupno na: https://urn.nsk.hr/urn:nbn:hr:217:530398. [Citirano: 22.01.2025.]

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Naslov (engleski)	Naegleria’s mitotic spindles are built from unique tubulins and highlight core spindle features
Autor	Katrina B. Velle
Autor	Andrew S. Kennard
Autor	Monika Trupinić
Autor	Arian Ivec
Autor	Andrew J.M. Swafford
Autor	Emily Nolton
Autor	Luke M. Rice
Autor	Iva M. Tolić
Autor	Lillian K. Fritz-Laylin
Autor	Patricia Wadsworth
Autorova ustanova	Sveučilište u Zagrebu Prirodoslovno-matematički fakultet (Fizički odsjek)
Znanstveno / umjetničko područje, polje i grana	PRIRODNE ZNANOSTI Fizika
Sažetak (engleski)	Naegleria gruberi is a unicellular eukaryote whose evolutionary distance from animals and fungi has made it useful for developing hypotheses about the last common eukaryotic ancestor. Naegleria amoebae lack a cytoplasmic microtubule cytoskeleton and assemble microtubules only during mitosis and thus represent a unique system for studying the evolution and functional specificity of mitotic tubulins and the spindles they assemble. Previous studies show that Naegleria amoebae express a divergent α-tubulin during mitosis, and we now show that Naegleria amoebae express a second mitotic α- and two mitotic β-tubulins. The mitotic tubulins are evolutionarily divergent relative to typical α- and β-tubulins and contain residues that suggest distinct microtubule properties. These distinct residues are conserved in mitotic tubulin homologs of the “brain-eating amoeba” Naegleria fowleri, making them potential drug targets. Using quantitative light microscopy, we find that Naegleria’s mitotic spindle is a distinctive barrel-like structure built from a ring of microtubule bundles. Similar to those of other species, Naegleria’s spindle is twisted, and its length increases during mitosis, suggesting that these aspects of mitosis are ancestral features. Because bundle numbers change during metaphase, we hypothesize that the initial bundles represent kinetochore fibers and secondary bundles function as bridging fibers.
Ključne riječi (engleski)
Jezik	engleski
Vrsta publikacije	Znanstveni rad - Izvorni znanstveni rad
Status objave	Objavljen
Vrsta recenzije	Recenziran - međunarodna recenzija
Verzija publikacije	Objavljena verzija rada (izdavačev PDF)
Naslov časopisa	Current Biology
Brojčani podaci	vol. 32, br. 6, str. 1247-1261.e6
p-ISSN	0960-9822
DOI	https://doi.org/10.1016/j.cub.2022.01.034
URN:NBN	urn:nbn:hr:217:530398
Datum objave publikacije	2022-03
Projekt	Šifra: IP-2019-04-5967 Naziv (hrvatski): Ravnoteža sila i momenta sila u diobenom vretenu Naziv (engleski): Balance of forces and torques in the mitotic spindle Kratica: SpindleForceBalance Voditelj: Nenad Pavin Pravna nadležnost: Hrvatska Financijer: Hrvatska zaklada za znanost Linija financiranja: Research Projects
Projekt	Šifra: KK.01.1.1.01.0004 Naziv (hrvatski): Provedba vrhunskih istraživanja u sklopu Znanstvenog centra izvrsnosti za kvantne i kompleksne sustave te reprezentacije Liejevih algebri Naziv (engleski): Implementation of top research within the Scientific Center of Excellence for Quantum and Complex Systems, and Representations of Lie Algebras Kratica: QuantiXLie Voditelj: Hrvoje Buljan Pravna nadležnost: eu Financijer: Europska komisija Linija financiranja: European Regional Development Fund
Projekt	Šifra: KK.01.1.1.04.0057 Naziv (hrvatski): Inovativni protokoli mikroskopije za interdisciplinarna istraživanja u biomedicini Naziv (engleski): Inovative microscopy protocols for interdisciplinary research in biomedicine Kratica: IPSted Voditelj: Iva Marija Tolić Pravna nadležnost: eu Financijer: Europska komisija Linija financiranja: European Regional Development Fund
Projekt	Šifra: 855158 Naziv (hrvatski): Molekularno podrijetlo aneuploidija u zdravim i bolesnim ljudskim tkivima Naziv (engleski): Molecular origins of aneuploidies in healthy and diseased human tissues Kratica: ANEUPLOIDY Voditelj: Iva Marija Tolić Pravna nadležnost: eu Financijer: Europska komisija
URL dokumenta	https://linkinghub.elsevier.com/retrieve/pii/S0960982222000458
Vrsta resursa	Tekst
Prava pristupa	Otvoreni pristup
Uvjeti korištenja
Javna napomena	© 2022 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Received: June 29, 2021. Received in revised form: November 2, 2021. Accepted: January 12, 2022. Published: February 8, 2022.
Datum i vrijeme pohrane	2024-08-22 20:05:05