Oblique circle method for measuring the curvature and twist of mitotic spindle microtubule bundles

Znanstveni rad - Izvorni znanstveni rad

• Autor: Arian Ivec
• Autor: Monika Trupinić
• Autor: Iva M. Tolić
• Autor: Nenad Pavin
• Autorova ustanova: Sveučilište u Zagrebu; Prirodoslovno-matematički fakultet; (Fizički odsjek)
• Znanstveno / umjetničko područje, polje i grana: PRIRODNE ZNANOSTI; Fizika

Sažetak (engleski)

The highly ordered spatial organization of microtubule bundles in the mitotic spindle is crucial for its proper functioning. The recent discovery of twisted shapes of microtubule bundles and spindle chirality suggests that the bundles extend along curved paths in three dimensions, rather than being confined to a plane. This, in turn, implies that rotational forces, i.e., torques, exist in the spindle in addition to the widely studied linear forces. However, studies of spindle architecture and forces are impeded by a lack of a robust method for the geometric quantification of microtubule bundles in the spindle. In this work, we describe a simple method for measuring and evaluating the shapes of microtubule bundles by characterizing them in terms of their curvature and twist. By using confocal microscopy, we obtain three-dimensional images of spindles, which allows us to trace the entire microtubule bundle. For each traced bundle, we first fit a plane and then fit a circle lying in that plane. With this robust method, we extract the curvature and twist, which represent the geometric information characteristic for each bundle. As the bundle shapes reflect the forces within them, this method is valuable for the understanding of forces that act on chromosomes during mitosis.

• Ključne riječi (engleski): mitotic spindle; chirality; twist; helicity; methods
• Jezik: engleski
• Vrsta publikacije: Znanstveni rad - Izvorni znanstveni rad
• Status objave: Objavljen
• Vrsta recenzije: Recenziran - međunarodna recenzija
• Verzija publikacije: Objavljena verzija rada (izdavačev PDF)
• Naslov časopisa: Biophysical Journal
• Brojčani podaci: vol. 120, br. 17, str. 3641-3648
• p-ISSN: 0006-3495
• DOI: https://doi.org/10.1016/j.bpj.2021.07.024
• URN:NBN: urn:nbn:hr:217:021477
• Datum objave publikacije: 2021-09
• Projekt: Šifra: 855158; Naziv (engleski): Molecular origins of aneuploidies in healthy and diseased human tissues; Kratica: ANEUPLOIDY; Pravna nadležnost: eu; Financijer: ERC-2019-SyG; Linija financiranja: Europska komisija
• Projekt: Šifra: 647077; Naziv (engleski): A new class of microtubules in the spindle exerting forces on kinetochores; Kratica: NewSpindleForce; Pravna nadležnost: eu; Financijer: EC; Linija financiranja: H2020
• Projekt: Šifra: IP-2019-04-5967; Naziv (hrvatski): Ravnoteža sila i momenta sila u diobenom vretenu; Naziv (engleski): Balance of forces and torques in the mitotic spindle; Kratica: SpindleForceBalance; Voditelj: Nenad Pavin; Pravna nadležnost: Hrvatska; Financijer: HRZZ; Linija financiranja: IP
• Projekt: Šifra: PZS-2019-02-7653; Naziv (hrvatski): Mehanizmi nastajanja snopova mikrotubula potrebni za sazrijevanje diobenog vretena; Naziv (engleski): Mechanisms of microtubule bundle formation required for spindle maturation; Kratica: SpindleForm; Voditelj: Iva Tolić; Pravna nadležnost: Hrvatska; Financijer: HRZZ; Linija financiranja: PZS
• URL dokumenta: https://linkinghub.elsevier.com/retrieve/pii/S0006349521006329
• Vrsta resursa: Tekst
• Prava pristupa: Otvoreni pristup
• Javna napomena: Copyright 2021 Biophysical Society. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). Submitted March 1, 2021, and accepted for publication July 27, 2021.
• Datum i vrijeme pohrane: 2023-07-28 20:45:39