Istraživanje interakcija pirenskog derivata poliamina spektroskopijom površinski pojačanog Ramanova raspršenja

master's thesis

• Title (english): Surface-enhanced Raman study of the interactions between pyrene derivative of polyamine and double-stranded polynucleotide
• Author: Petra Hudika
• Mentor: Adriana Kenđel (mentor)
• Committee member: Adriana Kenđel (predsjednik povjerenstva)
• Committee member: Biserka Prugovečki (član povjerenstva)
• Committee member: Ivana Biljan (član povjerenstva)
• Committee member: Snežana Miljanić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2021-02-26, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Spektroskopijom površinski pojačanog Ramanova raspršenja (SERS) istraţivane su
interakcije N,N,N-tri(8-piren-1-il-[3,7-diazaokt-1-il])amina (spoj 1) s polinukleotidima. Kao
metalni supstrati korišteni su svjeţe pripravljeni, koncentrirani i agregirani koloid. Spoj 1
najintenzivnije raspršuje zračenje pri koncentraciji 5×10−6 mol dm−3 u koncentriranom
koloidu te je navedeni supstrat korišten pri pripravi smjesa s polinukleotidima. Smjese su
pripremane na dva načina; kod prvog načina u koloid je dodan istraţivani spoj, a zatim
odabrani polinukleotid, dok se kod drugog načina prvo pripremio kompleks spoja 1 i
polinukleotida, koji je zatim dodan u koloidnu suspenziju. Dobiveni SERS spektri svih smjesa
nisu sadrţavali nove vrpce koje bi odgovarale vibracijama polinukleotida. Adsorpcija
molekula spoja 1 na nanočestice je prilično jaka i dodatak polinukleotida slabo utječe na
intenzitet i poloţaj vrpca. Kompleks spoj 1/polinukleotid daje slabe vrpce poliaminskog
derivata čak i pri velikom suvišku polinukleotida, vrlo vjerojatno zbog interkaliranja
pirenskog sustava izmeĎu parova baza DNA analoga, dok nevezani dio molekule spoja 1
ostaje blizu metalne površine.

Abstract (english)

Surface-enhanced Raman scattering (SERS) was used for studying interactions between pyrene
derivative of polyamine (1) and polynucleotides. Freshly prepared, concentrated and aggregated
colloids were used as metal substrates. Compound 1 scattered radiation most intensively at
concentration of 5 × 10−6 mol dm−3 in concentrated colloid, which was therefore used in the
preparation of mixtures with polynucleotides. The mixtures were prepared in two ways; firstly, 1
was added to the colloid, followed by adding selected polynucleotide, while in the second method
1/polynucleotide complex was prepared, and then mixed with the colloid. The obtained SERS
spectra of all mixtures showed lack of polynucleotides bands. The polyamine adsorption on the
silver was quite strong and the addition of polynucleotides poorly effected the bands intensity and
position. The compound 1/polynucleotide complex yielded weak bands originating from
compound 1, even with a large excess of polynucleotides, most likely due to intercalation of
pyrene part between base pairs of DNA analoges, while unbonded part of polyamine molecules
remain close to metal surface.

• Keywords: koloid; nanočestice srebra; SERS; piren; poliamin; polinukleotidi
• Keywords (english): colloid; SERS; silver nanoparticles; pyrene; polyamine; polynucleotides
• Language: croatian
• URN:NBN: urn:nbn:hr:217:617640
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2022-02-26
• Created on: 2021-03-18 09:53:23