Razvoj muških reproduktivnih organa Wistar štakora prenatalno izloženih alfa-cipermetrinu

master's thesis

• Title (english): Development of male reproductive organs in Wistar rats prenatally exposed to alfa-cypermethrin
• Author: Antonia Merčep
• Mentor: Anja Katić (mentor)
• Mentor: Nada Oršolić (komentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Marija Ivković (član povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-07-13, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Alfa-cipermetrin je sintetički piretroid tipa II, široke uporabe u poljoprivredi i kućanstvima.
Istraživanjima je dokazano da mnogi piretroidi mogu djelovati kao endokrini disruptori, ali za αcipermetrin to još uvijek nije dovoljno istraženo. Testisi su zbog reproduktivne važnosti i
osjetljivog razvoja dobar pokazatelj djelovanja endokrinih disruptora. Cilj ovog istraživanja bio je
po prvi puta istražiti utječe li peroralna prenatalna izloženost α-cipermetrinu na razvoj muških
reproduktivnih organa potomaka Wistar štakora. U tu svrhu provedene su histopatološke,
stereološke i imunohistokemijske analize proliferacijskog biljega histona H3 i apoptotičkog biljega
pocijepane kaspaze 3 na fetalnim testisima te analize brojnosti i vijabilnosti spermija i procjena
očuvanosti spermatogeneze u pubertalnim testisima štakora. S porastom doze α-cipermetrina
dolazi do deorganizacije intersticijskog prostora, gubitka cjelovitosti Leydigovih stanica i
značajnog smanjenja proliferacije Sertolijevih stanica. Prenatalna izloženost α-cipermetrinu ne
utječe na volumensku gustoću gonocita i Sertolijevih stanica i ne uzrokuje njihovu apoptozu u
fetalnim testisima, a kod mužjaka u pubertetu ne utječe na brojnost i vijabilnost spermija kao ni na
očuvanost spermatogeneze.

Abstract (english)

Alpha-cypermethrin is a synthetic pyrethroid type II widely used in agriculture and households.
Studies have shown that synthetic pyrethroids may act as endocrine disruptors, but for αcypermethrin it has not been investigated enough yet. Testes are a good indicator of endocrine
disruption due to their reproductive importance and sensitive development. The aim of this study
was to investigate for the first time whether oral prenatal exposure to α-cypermethrin affects the
development of male reproductive organs of Wistar rat offspring. For this purpose,
histopathological, stereological and immunohistochemical analyses of histone H3 proliferation
marker and apoptotic marker of cleaved caspase 3 in foetal testes and sperm number and viability
analyses as well as assessment of spermatogenesis maintenance in rat pubertal testes were
performed. Increase of α-cypermethrin dose leads to disorganisation of the interstitial space, loss
of Leydig cells integrity and significant reduction of the Sertoli cells proliferation. Prenatal
exposure to α-cypermethrin does not affect the volume density of gonocytes and Sertoli cells and
does not cause their apoptosis in foetal testes. Also, it does not affect the number and viability of
sperm as well as the maintenance of spermatogenesis in pubertal male rats.

• Keywords: endokrina disrupcija; histopatologija; imunohistokemija; sintetički piretroidi; štakori; testisi
• Keywords (english): endocrine disruption; histopathology; immunohistochemistry; synthetic pyrethroids; rats; testes
• Language: croatian
• URN:NBN: urn:nbn:hr:217:269343
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-07-19 09:32:33