Biomarkerski odgovori i akumulacija metala u mišićima štuke (Esox lucius) iz rijeke Mrežnice kao pokazatelji onečišćenja vode

master's thesis

• Title (english): Biomarker responses and accumulation of metals in the muscles of the northern pike (Esox lucius) from the Mrežnica River as indicators of water pollution
• Author: Ema Omrčen
• Mentor: Dušica Ivanković (mentor)
• Mentor: Jasna Lajtner (komentor)
• Committee member: Jasna Lajtner (predsjednik povjerenstva)
• Committee member: Petar Kružić (član povjerenstva)
• Committee member: Ivan Čanjevac (član povjerenstva)
• Committee member: Alan Moro (član povjerenstva)
• Committee member: Luka Valožić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2022-07-15, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Uslijed antropogenog djelovanja, posebice industrije, dolazi do onečišćenja vodenih ekosustava
metalima i ostalim zagađivalima, što ostavlja posljedice na vodenu biotu. Štuka (Esox lucius)
korištena je kao bioindikator u ovom radu. Uzorkovanje je provedeno na dvije postaje, referentnoj
(REF), smještenoj oko 2 km uzvodno od Duge Rese i onečišćenoj, smještenoj neposredno ispred
nekadašnje tvornice tekstila u Dugoj Resi (DRT). Analizirane su koncentracije metala i metaloida
u mišiću štuke te su praćeni odgovori biomarkera acetilkolinesteraze, ukupnog glutationa,
malondialdehida te ukupnih proteina kako bi se dobio uvid o izloženosti štuka onečišćenju.
Koncentracije Tl, Se, Mn i Rb u mišiću bile su značajno više na REF postaji, dok su koncentracije
toksičnih metala Cd, Pb, Ag, Cr i Ni bile ispod granice detekcije. Razlike u veličinama riba utjecale
su na dobivene razlike između postaja za elemente Tl, Mn i As. Ustanovljena je značajno viša
koncentracija Bi kod jedinki ženskog, a Co kod muškog spola. Jedino su koncentracije biomarkera
malondialdehida pokazale značajnu razliku između postaja, s višim vrijednostima na DRT postaji.
Rezultati dobiveni ovim radom donose nove spoznaje o utjecaju industrijskih aktivnosti na vodenu
biotu, s naglaskom na ribe te njihove sposobnosti bioakumulacije metala.

Abstract (english)

Anthropogenic impact, especially industry, leads to pollution of aquatic ecosystems by metals and
other pollutants which effect water biota. Northern pike (Esox lucius) is used as the bioindicator
in this study. Northern pikes were sampled at two sites, at the reference site located 2 km upstream
of Duga Resa (REF) and at the polluted site located directly in front of former textile industry in
Duga Resa (DRT). There were analysed the concentrations of metals and metalloids in the muscle
of the northern pike and the responses of the biomarkers acetylcholinesterase, total glutathione,
malondialdehyde and total proteins were monitored to gain insight into northern pike exposure to
pollution. Concentrations of Tl, Se, Mn and Rb were significantly elevated on REF site while the
concentrations of toxic metals Cd, Pb, Ag, Cr and Ni were below the limit of detection. Differences
in fish sizes on REF and DRT sites significantly effected the obtained differences between sites
for elements Tl, Mn and As. Statistically significant higher concentration of Bi was found in
females, while Co had higher concentration in males. Among analysed biomarkers, only
malondialdehyde concentrations showed a significant difference between sites, with significantly
higher values at the DRT site. Results obtained in this study bring new insights into the impact of
industrial activities on water biota, with an emphasis on fish and their abilities of metal
bioaccumulation.

• Keywords: : bioakumulacija; industrijsko onečišćenje; ribe; spol; veličina
• Keywords (english): bioaccumulation; industrial pollution; fish; sex; size
• Language: croatian
• URN:NBN: urn:nbn:hr:217:987479
• Study programme: Title: Enviromental Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Embargoed access; Embargo expiration date: 2024-07-15
• Created on: 2022-07-19 10:32:23