Antibakterijsko djelovanje nanočestica metalnih oksida na klinoptilolitu

master's thesis

• Title (english): Antibacterial activity of metal oxide nanoparticles deposited onto clinoptilolite
• Author: Tea Škrtić
• Mentor: Jasna Hrenović (mentor)
• Committee member: Jasna Hrenović (predsjednik povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Mladen Kerovec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2012-05-23, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Zeoliti su prirodni minerali koji se istražuju u svrhu smanjenja raznih oblika onečišćenja. Sorbiraju ione teških metala iz vodenih otopina, te nastaje produkt koji ima antibakterijsko djelovanje. Ispitan je učinak prirodnog zeolita na bakterije E. coli, te nanočestica Cu2O i ZnO deponiranih na zeolitu na čistu kulturu i na nativne populacije bakterija E. coli. Pokusi su izvođeni u autoklaviranom sekundarnom efluentu iz uređaja za biološko pročišćavanje otpadne vode. Prirodni zeolit nema antibakterijsko djelovanje već imobilizira bakterije na svojoj površini (1,83±1,99 x 107 CFU/g), no nanočestice metalnih oksida imaju dobro antibakterijsko djelovanje na E. coli. Nanočestice Cu2O imaju bolju antibakterijsku aktivnost od nanočestica ZnO. Bolja antibakterijska aktivnost utvrđena je na nativnim populacijama (nakon 1 sata obje vrste nanočestica postižu 100% inhibicije i zadržavaju ju i nakon 24 sata) nego na čistim kulturama bakterija (nakon 24 sata nanočestice Cu2O inhibiraju 87,24% bakterija, a nanočestice ZnO 53,72% bakterija). Zeolit se u svrhu pročišćavanja otpadnih voda može koristiti 5 dana nakon čega značajno gubi antibakterijska svojstva.

Abstract (english)

Zeolites are natural minerals that are widely investigated in the reduction of various forms of pollution. Zeolites sorb heavy metal ions from aqueous solution and form a product that has an antibacterial effect. We examined the effect of natural zeolite in E. coli, and Cu2O and ZnO nanoparticles deposited onto the zeolite in pure culture and in the native populations of the bacteria E. coli. Experiments were performed in autoclaved secondary effluent from the biological wastewater treatment plant. Natural zeolite has no antibacterial activity, but immobilizes bacteria on its surface (1,83±1,99 x 107 CFU/g), whereas metal oxide nanoparticles have good antibacterial activity on E. coli. Cu2O nanoparticles have better antibacterial activity than ZnO nanoparticles. Better antibacterial activity was found in native populations (after 1 hour both types of nanoparticles reached 100% inhibition and retain it also after 24 hours) than on pure cultures of bacteria (after 24 hours of Cu2O nanoparticles inhibited 87.24% of bacteria, and ZnO nanoparticles 53.72% of bacteria). Zeolite in wastewater treatment can be used for 5 days after which it loses antibacterial properties.

• Keywords: bakterija; klinoptilolit; nanočestice; bakar; cink; otpadna voda
• Keywords (english): bacteria; clinoptilolite; nanoparticles; copper; zinc; wastewater
• Language: croatian
• URN:NBN: urn:nbn:hr:217:704618
• Study programme: Title: Ecology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra ekologije i zaštite prirode (magistar/magistra ekologije i zaštite prirode)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 11:49:01