Genotoksični i citotoksični učinci cisplatine u kombinaciji s anastetikom halotanom na Ehrlichov ascitesni tumor u miša

master's thesis

• Title (english): Genotoxic and cytotoxic effects of cisplatin in combination with anesthetics halothane on Ehrlichov ascites tumor in mice
• Author: Maja Šutić
• Mentor: Nada Oršolić (mentor)
• Committee member: Nada Oršolić (predsjednik povjerenstva)
• Committee member: Sandra Radić Brkanac (član povjerenstva)
• Committee member: Mirjana Kalafatić (član povjerenstva)
• Committee member: Zoran Tadić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; Zagreb
• Defense date and country: 2012-07-17, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES

Abstract

Istražen je kombinirani učinak cisplatine i anestetika halotana na DNA tumorskih stanica i njihov učinak na nepovratna oštećenja EAT stanica koja vode do apoptoze u in vivo uvjetima. Miševima korištenim u istraživanju intraperitonealno je injicirano 2x106 EAT stanica, potom su bili izloženi djelovanju inhalirajućeg halotana, cisplatine, i djelovanju halotana nakon injiciranja cisplatine u peritonealnu šupljinu tijekom tri dana. Genotoksični učinak inhalacijskog halotana, samog ili u kombinaciji sa cisplatinom, na stanice Ehrlichovog ascitesnog tumora u miševa, procijenjen je pomoću alkalnog komet testa. Postotak apoptotičnih EAT stanica procijenjen je protočnim citometrom. Uočeno je da halotan ima jak genotoksični učinak na tumorske stanice in vivo i da uzrokuje smanjenje broja živih EAT stanica u peritonealnoj šupljini. Cisplatina uzrokuje apoptozu velikog broja EAT stanica (41,14%) i smanjuje broj živih EAT stanica u trbušnoj šupljini miševa. Kombinacija halotana i cisplatine rezultira većim brojem živih EAT stanica u odnosu na skupinu obrađenu samo cisplatinom.

Abstract (english)

In this study, I investigated combined effecs of cisplatin and anesthetic halotan on DNA damage in tumor cell, and their effects on irreversible cell damage leading to apoptosis in vivo. Mices used in this study were injected with 2x106 EAT cells into peritoneal cavity. Then were exposed to anesthetic halotan, cisplatin, and subjected to combined treatment of halotan after cisplatin for 3 days. Genotoxic effects of halotan on EAT cells alone, or in combined applicaton with cisplatin, was estimated by using the alkaline comet assay. The percentage of EAT cell apoptosis was estimated by flow cytometry. Halothan caused strong genotoxic effects on tumor cells in vivo, and has decreased the number of living EAT cells in peritoneal cavity. Cisplatin caused apoptosis of EAT cells (41.14%) and decreased the number of living EAT cells. Combined halothan and cisplatin increased the number of living cells in the peritoneal cavity, compared to cisplatin treatment alone.

• Keywords: kemoterapeutik; anestezija; apoptoza; oštećenja DNA; tumor
• Keywords (english): antitumor drug; anesthesia; apoptosis; DNA damage; tumor
• Language: croatian
• URN:NBN: urn:nbn:hr:217:521620
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 12:14:56