Uloga polimorfizma gena DRD4 i aktivnosti enzima MAO u razvoju ovisnosti o nikotinu

master's thesis

• Title (english): The role of DRD4 gene polymorphism and activity of MAO enzyme in development of nicotine addiction
• Author: Mirjana Babić
• Mentor: Nela Pivac (mentor)
• Mentor: Dubravka Hranilović (sumentor)
• Committee member: Nela Pivac (predsjednik povjerenstva)
• Committee member: Dubravka Hranilović (član povjerenstva)
• Committee member: Maja Matulić (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2011-12-19, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Ovisnost o nikotinu izazvana pušenjem nastaje kao produkt socijalnih, okolišnih i genetičkih čimbenika. Geni dopaminergičkog sustava su povezani s pušenjem. Literatura pokazuje povezanost i/ili nepovezanost polimorfizma varijabilnog broja ponavljanja (VNTR) u trećem egzonu gena dopaminskog receptora tipa 4 (DRD4) i pušenja te smanjenu ili nepromijenjenu aktivnost trombocitne monoaminooksidaze tipa B (MAO-B) u pušača. Cilj ovog rada bio je odrediti postoji li u hrvatskoj populaciji povezanost VNTR-a u genu DRD4 te aktivnosti MAO-B s pušenjem. Genotipizirano je 565 ispitanika, a aktivnost MAO-B određena je u 302 ispitanika. Nije uočena značajna razlika u učestalosti dugih i kratkih alela DRD4 između pušača i nepušača. U muških pušača je pronađena snižena aktivnost MAO-B u odnosu na nepušače, dok u ženskih ispitanika nisu pronađene razlike u aktivnosti tog enzima. Rezultati ovog rada nisu potvrdili hipotezu o povezanosti genotipa DRD4 i pušenja, dok je hipoteza o sniženoj aktivnosti MAO-B kod pušača potvrđena za muške, ali ne i za ženske ispitanike zbog premalog uzorka.

Abstract (english)

Nicotine addiction, related to smoking, develops as a product of social, environmental and genetic factors. Dopaminergic system genes are associated with smoking. Literature shows association and/or lack of association between variable number of tandem repeats (VNTR) polymorphism located in the third exon of D4 dopamine receptor gene (DRD4) and smoking, and reduced or unaltered platelet monoamine oxidase type B (MAO-B) activity in smokers. The aim of this study was to determine whether VNTR in DRD4 gene and MAO-B activity are associated with smoking in Croatian population. Genotyping was done in 565 subjects, while MAO-B activity was determined in 302 subjects. There was no significant difference in distribution of the long and short DRD4 alleles between smokers and non-smokers. MAO-B activity was decreased in male smokers compared to male non-smokers, while in female subjects there were no differences in the activity of this enzyme. The results of this study failed to confirm the hypothesis about the association between DRD4 genotype and smoking, while the hypothesis of the reduced platelet MAO-B activity in smokers was confirmed in male, but not in female subjects because of the small sample.

• Keywords: Dopaminski receptor tipa 4; monoaminooksidaza tipa B; ovisnost o nikotinu
• Keywords (english): D4 dopamine receptor (DRD4); monoamine oxidase type B (MAO-B); nicotine addiction
• Language: croatian
• URN:NBN: urn:nbn:hr:217:738066
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-02 12:35:21