Razvoj spektrometrijskih analitičkih metoda za određivanje selenija u biljnim dodatcima prehrani

doctoral thesis

• Title (english): Development of analytical spectrometry methods for determination of selenium in herbal dietary supplements
• Author: Anica Benutić
• Mentor: Sanda Rončević (mentor)
• Committee member: Iva Juranović Cindrić (predsjednik povjerenstva)
• Committee member: Darija Klinčić (član povjerenstva)
• Committee member: Jasna Jurasović (član povjerenstva); MBZ: 160496
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2022-11-16, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry
• Universal decimal classification (UDC): 54 - Chemistry. Crystallography. Mineralogy

Abstract

U ovom radu razvijen je postupak određivanja sadržaja selenija i odabranih elemenata u osamdeset i sedam različitih uzoraka biljnih dodataka prehrani koji su prikupljeni na hrvatskom tržištu u obliku kapsula, praha, džema ili tekućine. Metodom elektrotermičke atomske apsorpcije uz hvatanje in situ razvijenog hidrida na iridijem obloženoj grafitoj kiveti (HG-ETAAS) izmjerene su koncentracije selenija. Primjenom spektrometrije masa uz induktivno spregnutu plazmu (ICP-MS) izmjereni su sadržaji... More dvadeset i četiri elementa (Se, Al, As, B, Ba, Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Mg, Mn, Mo, Na, Ni, Pb, Sb, Sr, Tl, V, Zn) u svim uzorcima. Mjerne sposobnosti dvije metode nisu se značajno razlikovale i obje metode jednako ispunjavaju zahtjeve za određivanje sadržaja selenija u biljnim dodatcima prehrani. Multivarijatnim statističkim alatima, analizom glavnih komponenata (PCA) i hijerarhijskom analizom klastera (HCA), klasificirani su dobiveni elementni profili i omogućena je vizualizacija i korelacija specifičnih elementnih profila ispitivanih uzoraka. Iz dobivenih elementnih profila potvrđeni su posebni utjecaji botaničkih, okolišnih i/ili proizvodnih uvjeta na elementni profil biljnih dodataka prehrani. Less

Abstract (english)

In this work, the determination of selenium and selected elements in eighty-seven different herbal dietary supplements present at Croatian market in the forms of capsules, powder, tablets, jams, and liquors were analyzed. Electrothermal atomic absorption was applied with in situ trapping of generated hydride on iridium coated graphite cuvette (HG-ETAAS) for the determination of selenium. By inductively coupled plasma mass spectrometry (ICPMS), the twenty-four elements (Se, Al, As, B, Ba,... More Ca, Cd, Co, Cr, Cu, Fe, Hg, K, Mg, Mn, Mo, Na, Ni, Pb, Sb, Sr, Tl, V, Zn) were determined in all samples. Measurement capabilities of both methods were not significantly different and both methods are applicable for selenium analysis in herbal dietary supplements. By using multivariate statistical tools, principal component analysis (PCA) and hierarchical cluster analysis (HCA), elemental profiles of samples were classified. This allowed visualization and correlation of specific elemental profiles of examined samples. Distinctive features of herbal dietary supplements with peculiar influences of botanical, environmental and/or production conditions on their elemental profile were highlighted. Less

• Keywords: biljni dodatci prehrani; elektrotermička atomska apsorpcija s tehnikom generiranja hidrida; elementni profili; multivarijantna statistička analiza; selenij; spektrometrija masa uz induktivno spregnutu plazmu
• Keywords (english): electrothermal atomic absorption with hydride generation technique; elemental profiling; herbal dietary supplements; inductively coupled plasma mass spectrometry; multivariate statistical analysis; selenium
• Language: croatian
• URN:NBN: urn:nbn:hr:217:567483
• Promotion: 2022
• Study programme: Title: Doctoral study in chemistry; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija (doktor/doktorica znanosti, područje prirodnih znanosti, polje kemija)
• Type of resource: Text
• Extent: 99 str. ; 30 cm
• File origin: Born digital
• Access conditions: Open access
• Created on: 2022-11-24 13:55:10