Procjena genotoksičnog učinka pesticida imazalila primjenom alkalnog komet-testa i mikronukleus-testa u miša

master's thesis

• Title (english): Evaluation of genotoxic effect of pesticide Imazalil using alkaline comet assay and micronucelus assay in mice
• Author: Leana Šibić
• Mentor: Vesna Benković (mentor)
• Committee member: Vesna Benković (predsjednik povjerenstva)
• Committee member: Alan Moro (član povjerenstva)
• Committee member: Danijel Orešić (član povjerenstva)
• Committee member: Maria Špoljar (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2011-07-08, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Imazalil je fungicid, koji je široko rasprostranjen u poljoprivredi, koristi se za suzbijanje gljivica na voću, povrću i ukrasnom bilju. Također se koristi u tretmanima sjemenja nakon žetve te u sprečavanju propadanja citrusnog voća i banana prilikom skladištenja. Cilj ovog istraživanja bio je utvrditi genotoksični učinak ovog pesticida na tkiva pojedinih organa (slezena, limfni čvorovi, timus i krv) Swiss miševa. Plan pokusa bio je sukladan odredbama OECD 407. Životinje su raspoređene u 2 skupine (kontrolna i tretirana, u svakoj skupini je po 5 muških i 5 ženskih životinja). Eksperimentalne životinje tretirane su NOAEL dozom pesticida Imazalila (10 mg/kg) koji je svakodnevno primjenjivan oralno tijekom 28 dana, nakon čega je izvršen alkalni komet test na stanicama krvi, limfnih čvorova, slezene i timusa i brojanje mikronukleusa u perifernoj krvi. Imazalil je inducirao značajno povećanje učestalosti mikronukleusa kod oba spola. Uočili smo povećanje vrijednosti duljine repa kometa u tretiranoj skupini životinja na stanice četiri ispitivana organa u odnosu na kontrolnu skupinu. Značajno povećanje vrijednosti duljine repa u stanicama krvi, slezene, timusa i limfnih čvorova ukazuje na jači genotoksični učinak Imazalila u ženskih nego u muških miševa.

Abstract (english)

Imazalil is a fungicide that is widely used in agriculture to control fungi on fruits, vegetables and ornamentals. It is also used in treatment of seeds after harvest and to prevent deterioration of citrus fruits and bananas during storage. The aim of this research was to determine the genotoxic effects of this pesticide in tissues of some organs (spleen, lymph nodes, thymus and blood) in Swiss mice. The plan of experiment was consistent with written standard OECD 407. The animals were classified in two groups (control and treated, in each group by 5 male and 5 female animals). Experimental animals were exposed to the NOAEL dose of the pesticide Imazalil (10 mg / kg) which was daily administered orally for 28 days, after which we made alkaline comet assay performed on cells of the blood, lymph nodes, spleen and thymus, and counting of micronuclei in peripheral blood. Imazalil induced significant increase in the frequency of micronuclei in both sexes. We found increased values of comet tail length in the treated group of animals in cells of four examined organs in relation to the control group. The significant increase in the value of tail length in blood cells, spleen, thymus and lymph nodes indicates a stronger genotoxic effect of Imazalil in the female than in male mice.

• Keywords: Imazalil; genotoksičnost; Swiss-miš; alkalni komet test; retikulocit; mikronukleus test in vivo
• Keywords (english): Imazalil; genotoxic; Swiss-mice; alkaline comet assay; reticulocytes; micronucleus test in vivo
• Language: croatian
• URN:NBN: urn:nbn:hr:217:889771
• Study programme: Title: Enviromental Sciences; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra struke znanosti o okolišu (magistar/magistra struke znanosti o okolišu)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-03 10:25:13