Učinak indol-3-maslačne kiseline na oksidacijski stres u u vinovoj lozi (Vitis vinifera L.) zaraženoj fitoplazmama

master's thesis

• Title (english): The effect of indol-3-butyric acid on oxidative stress in phytoplasma-infected grapevine (Vitis vinifera L.)
• Author: Andrijana Kozina
• Mentor: Mirna Ćurković Perica (mentor)
• Committee member: Mirna Ćurković Perica (predsjednik povjerenstva)
• Committee member: Mirta Tkalec (član povjerenstva)
• Committee member: Gordana Lacković-Venturin (član povjerenstva)
• Committee member: Biljana Balen (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2010-10-12, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Fitoplazmama zaražena i zdrava vinova loza (Vitis vinifera L.) tretirana je folijarno indol-3-maslačnom kiselinom (IBA) u koncentracijama 0,5, 1,0 i 1,5 g/trsu. Metodom "ugniježdene" lančane reakcije polimerazom (PCR) određivana je prisutnost fitoplazmatskog gena za 16S rRNA prije i nakon tretmana IBA-om. Također, mjereni su pokazatelji oksidacijskog stresa kako bi utvrdili utječu li reaktivni oblici kisika na oporavak zaražene loze. Rezultati PCR-a ukazuju na prisutnost fitoplazmi u samo tri uzorka od šesnaest u kojima je prethodno potvrđena zaraza. IBA (1,0 g/trsu) je uzrokovala porast vodikovog peroksida dok se stopa lipidne peroksidacije i sadržaj ukupnih fenolnih spojeva nisu značajno promijenili nakon tretmana IBA-om. Primijećena je i povećana aktivnost peroksidaze 3 mjeseca nakon tretmana IBA-om, ali ne i neposredno nakon tretmana. Dobiveni rezultati pokazuju da IBA može djelovati na određene pokazatelje oksidacijskog stresa koji se mogu povezati s oporavkom fitoplazmama zaražene loze, no interpretacija rezultata je složena i potrebna su daljnja istraživanja.

Abstract (english)

Phytoplasma-infected and healthy grapevine (Vitis vinifera L.) was treated foliary with indole-3-butyric acid (IBA) in concentrations of 0.5, 1.0 and 1.5 g/vine. Presence of the phytoplasma gene for 16S rRNA before and after the treatment was determined by nested polymerase chain reaction (PCR). Oxidative stress markers were measured to reveal whether reactive oxygen species affect phytoplasma-recovery in grapevine. PCR results confirmed the presence of phytoplasma in only three of sixteen samples, that were previously confirmed as phytoplasma infected. IBA (1.0 g/vine) caused a rise in hydrogen peroxide, while lipid peroxidation and phenolic content was not significant changed after treatments. Peroxidase activity increased three months after the treatment with IBA, but not immediately after the treatment. Obtained results indicate that IBA may act on specific indicators of oxidative stress which could be correlated with the phytoplasma-recovery in grapevine, but the interpretation of the results is complex and further research is required.

• Keywords: fitoplazme; IBA; vinova loza (Vitis vinifera L.); oksidacijski stres
• Keywords (english): phytoplasma; IBA; grapevine (Vitis vinifera L.); oxidation stress
• Language: croatian
• URN:NBN: urn:nbn:hr:217:778022
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-04 07:31:57