Metilacija citozina u genima MGMT i RASSF1A u pacijenata s karcinomom testisa

master's thesis

• Title (english): CpG metilation of MGMT and RASSF1A genesis in patients with testicular carcinoma
• Author: Marina Srbić
• Mentor: Vlatka Zoldoš (mentor)
• Committee member: Vlatka Zoldoš (predsjednik povjerenstva)
• Committee member: Sandra Radić Brkanac (član povjerenstva)
• Committee member: Martina Šeruga Musić (član povjerenstva)
• Committee member: Nataša Bauer (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016-03-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Karcinom testisa (TC) predstavlja oko 1% svih novodijagnosticiranih zloćudnih tumora muške populacije. Smatra se da nastaje iz primordijalnih zametnih stanica u ranom embrionalnom razvoju kada se roditeljski obrasci metilacije DNA brišu, te se uspostavlja totipotentnost stanica. Diferencijacijom podskupina TC-a postupno se povećava stupanj DNA metilacije zbog uspostavljanja metilacijskog obrasca. Cilj ovog istraživanja je bila analiza DNA metilacije unutar CpG otoka gena MGMT i RASSF1A iz periferne krvi pacijenata s karcinomom testisa i zdravih ljudi. U tu svrhu je korištena metoda pirosekvenciranja bisulfitno konvertirane DNA za analizu stupnja metilacije svakog pojedinog CpG dinukleotida unutar fragmenata gena MGMT i RASSF1A. Rezultati ne pokazuju statistički značajnu promjenu u CpG metilaciji gena MGMT između pacijenata i zdravih osoba. Međutim, CpG metilacija u analiziranim fragmentima unutar gena RASSF1A je statistički značajno promijenjena u pacijenata u odnosu na zdrave osobe, te ukazuje na potencijal CpG metilacije ovog gena analizirane u perifernoj krvi, kao dijagnostičkog biljega.

Abstract (english)

Testicular cancer (TC) represents about 1% of all new diagnosed malignant cancer in male population. TC are believed to arise from primordial germ cells in early embrional development when DNA methylation and parental imprints are erased and totipotency is restored. With differentiation of subgroups of TC the level of DNA methylation gradually increases. The main goal of this experiment was to analyse methylation level inside CpG islands of the MGMT and RASSF1A genes in blood samples of patients with testicular cancer and healthy individuals. For this purpose, methylation status was determined for each CpG sites of bisulfite converted DNA using pyrosequencing method. The results are not showing statistically significant change in CpG methylation within the analysed fragments of MGMT gene in patients with testicular cancer compared to healthy individuals. However, for RASSF1A the results show statistically significant change in CpG methylation analysed in patients with testicular cancer compared with healthy individuals, thus revealing the potential of this gene as a methylation biomarker in periferal blood.

• Keywords: karcinom testisa; metilacija DNA; CpG otok; gen MGMT; gen RASSF1A; molekularni biljeg iz krvi
• Keywords (english): testicular cancer; DNA methylation; CpG island; MGMT; RASSF1A; blood biomarker
• Language: croatian
• URN:NBN: urn:nbn:hr:217:051072
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-05-12 08:33:11