Ekotoksikološka karakterizacija otpadnih voda i aktivnoga mulja upotrebom biokemijskih i histopatoloških biomarkera

doctoral thesis

• Title (english): Ecotoxicological characterization of waste waters and activated sludge using biochemical and histopathological biomarkers
• Author: Sanja Babić
• Mentor: Roberta Sauerborn Klobučar (mentor)
• Mentor: Mirjana Pavlica (sumentor)
• Committee member: Jasna Hrenović (predsjednik povjerenstva)
• Committee member: Natalija Topić-Popović (član povjerenstva)
• Committee member: Ana Bielen (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 57 - Biological sciences. Physical anthropology. Biodiversity

Abstract

Velike količine otpadnih voda i mulja, kao nusprodukata uređaja za pročišćavanje otpadnih voda (UPOV), u konačnici bivaju ispuštene u slatkovodne, morske ili kopnene ekosustave. Nusprodukti UPOV-a često sadrže različita organska, anorganska i mikrobiološka zagađivala koja imaju mogućnost akumulacije u tlu, sedimentu, usjevima, kao i prijenosa unutar hranidbenih lanaca kroz različite trofičke nivoe, predstavljajući tako prijetnju i ljudskom zdravlju. Unatoč činjenici da se u većini slučajeva radi o kompleksnoj smjesi različitih spojeva, državne i međunarodne zakonske odredbe preporučaju praćenje svega nekoliko odabranih zagađivala te su većinom bazirane na određivanju kemijskog sastava. S obzirom na to da potencijalna toksičnost i biodostupnost može biti određena isključivo upotrebom različitih bioindikatorskih organizama, istraživanje opisano u ovom doktorskom radu usmjereno je ka širem ekološkom pristupu, pružajući tako sveobuhvatan i jasniji uvid u mogućnost onečišćenja kopnenih i vodenih ekosustava. Predstavljen doktorski rad prikazuje i tumači rezultate niza istraživanja otpadnih voda, tekućeg i aktivnog te dehidriranog otpadnog mulja s odlagališta, provedenih na embrijima zebrice Danio rerio i gujavicama vrste Eisenia fetida. U procjeni toksičnosti upotrijebljen je niz biokemijskih i histopatoloških biomarkera na različitim razinama biološke organizacije (substanična, stanična i tkivna razina). Integracijom krajnjih bioloških učinaka ispitivanih na dva modelna organizma (zebricama i gujavicama), ovaj doktorski rad ne predstavlja samo procjenu efikasnosti UPOV-a Virovitica, već pruža temelj za praćenje onečišćenja otpadnih voda i mulja.

Abstract (english)

Large amounts of sewage effluents and sludges produced by municipal wastewater treatment plant (WWTP) ultimately result in discharge of treated byproducts in receiving freshwaters, marine or soil environments. WWTP’s byproducts often contain various organic, inorganic and microbiological contaminants, which could be accumulated in soil, sediment, crops, and transferred to the food chain on different trophic levels, having consequences also on human health. Despite the fact that sewage effluents and sludges are very complex mixtures, national and international legislation provide recommendations for monitoring of only few selected contaminants and are based mostly on chemical composition. Regarding to the fact that potential toxicity and bioavailability can only be determined using various bioindicators, the research described within this thesis has focussed on a broader ecological approach, providing a comprehensive and clearer insight into the possibility of terrestrial and aquatic ecosystems contamination. Presented thesis consists of a series of studies on wastewater, activated and dehydrated sludges, using two model organisms - zebrafish Danio rerio embryos and earthworm Eisenia fetida. In assessing the toxicity, several biochemical and histopathological biomarkers at various levels of biological organisation (sub-cellular, cellular and tissue level) were conduced. By integrating different biological endpoints within two test organisms, this thesis not only provides determination of efficiency of WWTP Virovitica, but also implements a basis for wastewater and sewage sludge pollution control.

• Keywords: otpadne vode; aktivni mulj; gujavica; E. fetida; embrij zebrice; D. rerio; testovi toksičnosti
• Keywords (english): waste water; activated sludge; earthworm; E. fetida; zebrafish embryo; D. rerio; toxicity tests
• Language: croatian
• URN:NBN: urn:nbn:hr:217:754211
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-12 09:36:15