prikaz prve stranice dokumenta Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment
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Scientific paper - Original scientific paper
Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment
Cell Reports, 40 (2022), 5; 111169. https://doi.org/10.1016/j.celrep.2022.111169
Risteski, Patrik; Božan, Domagoj; Jagrić, Mihaela; Bosilj, Agneza; Pavin, Nenad; Tolić, Iva M.

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Risteski, P., Božan, D., Jagrić, M., Bosilj, A., Pavin, N. & Tolić, I. M. (2022). Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment. Cell Reports, 40. (5). doi: 10.1016/j.celrep.2022.111169

Risteski, Patrik, et al. "Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment." Cell Reports, vol. 40, no. 5, 2022. https://doi.org/10.1016/j.celrep.2022.111169

Risteski, Patrik, Domagoj Božan, Mihaela Jagrić, Agneza Bosilj, Nenad Pavin and Iva M. Tolić. "Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment." Cell Reports 40, no. 5 (2022). https://doi.org/10.1016/j.celrep.2022.111169

Risteski, P., et al. (2022) 'Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment', Cell Reports, 40(5). doi: 10.1016/j.celrep.2022.111169

Risteski P, Božan D, Jagrić M, Bosilj A, Pavin N, Tolić IM. Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment. Cell Reports [Internet]. 2022 August [cited 2024 November 14];40(5). doi: 10.1016/j.celrep.2022.111169

P. Risteski, D. Božan, M. Jagrić, A. Bosilj, N. Pavin and I. M. Tolić, "Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment", Cell Reports, vol. 40, no. 5, August 2022. [Online]. Available at: https://urn.nsk.hr/urn:nbn:hr:217:995052. [Accessed: 14 November 2024]

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Metadata
Title (english)Length-dependent poleward flux of sister kinetochore fibers promotes chromosome alignment
AuthorPatrik Risteski
AuthorDomagoj Božan
AuthorMihaela Jagrić
AuthorAgneza Bosilj
AuthorNenad Pavin
AuthorIva M. Tolić
Author's institutionUniversity of Zagreb
Faculty of Science
(Department of Physics)
Scientific / art field,
discipline and subdiscipline		NATURAL SCIENCES
Physics
Abstract (english)
Chromosome alignment at the spindle equator promotes proper chromosome segregation and depends on pulling forces exerted at kinetochore fiber tips together with polar ejection forces. However, kinetochore fibers are also subjected to forces driving their poleward flux. Here we introduce a flux-driven centering model that relies on flux generated by forces within the overlaps of bridging and kinetochore fibers. This centering mechanism works so that the longer kinetochore fiber fluxes faster than the shorter one, moving the kinetochores toward the center. We develop speckle microscopy in human spindles and confirm the key prediction that kinetochore fiber flux is length dependent. Kinetochores are better centered when overlaps are shorter and the kinetochore fiber flux slower than the bridging fiber flux. We identify Kif18A and Kif4A as overlap and flux regulators and NuMA as a fiber coupler. Thus, length-dependent sliding forces exerted by the bridging fiber onto kinetochore fibers support chromosome alignment.
Keywords (english)
Languageenglish
Publication typeScientific paper - Original scientific paper
Publication statusPublished
Peer reviewPeer review - international
Publication versionPublished version
Journal titleCell Reports
Numberingvol. 40, no. 5, 111169
p-ISSN2211-1247
DOIhttps://doi.org/10.1016/j.celrep.2022.111169
URN:NBNurn:nbn:hr:217:995052
Publication2022-08
ProjectNumber: 855158
Title (croatian): Molekularno podrijetlo aneuploidija u zdravim i bolesnim ljudskim tkivima
Title (english): Molecular origins of aneuploidies in healthy and diseased human tissues
Acronym: ANEUPLOIDY
Leader: Iva Marija Tolić
Jurisdiction: eu
ProjectNumber: 647077
Title (croatian): Nova klasa mikrotubula u vretenu koja djeluje silama na kinetohore
Title (english): A new class of microtubules in the spindle exerting forces on kinetochores
Acronym: NewSpindleForce
Leader: Iva Marija Tolić
Jurisdiction: eu
ProjectNumber: PZS-2019-02-7653
Title (croatian): Mehanizmi nastajanja snopova mikrotubula potrebni za sazrijevanje diobenog vretena
Title (english): Mechanisms of microtubule bundle formation required for spindle maturation
Acronym: SpindleForm
Leader: Iva Marija Tolić
Jurisdiction: Croatia
Funding stream: Programme Research Cooperability
ProjectNumber: IP-2019-04-5967
Title (croatian): Ravnoteža sila i momenta sila u diobenom vretenu
Title (english): Balance of forces and torques in the mitotic spindle
Acronym: SpindleForceBalance
Leader: Nenad Pavin
Jurisdiction: Croatia
Funding stream: Research Projects
ProjectNumber: KK.01.1.1.04.0057
Title (croatian): Inovativni protokoli mikroskopije za interdisciplinarna istraživanja u biomedicini
Title (english): Inovative microscopy protocols for interdisciplinary research in biomedicine
Acronym: IPSted
Leader: Iva Marija Tolić
Jurisdiction: eu
Funding stream: European Regional Development Fund
ProjectNumber: KK.01.1.1.01.0004
Title (croatian): Provedba vrhunskih istraživanja u sklopu Znanstvenog centra izvrsnosti za kvantne i kompleksne sustave te reprezentacije Liejevih algebri
Title (english): Implementation of top research within the Scientific Center of Excellence for Quantum and Complex Systems, and Representations of Lie Algebras
Acronym: QuantiXLie
Leader: Hrvoje Buljan
Jurisdiction: eu
Funding stream: European Regional Development Fund
Document URLhttps://linkinghub.elsevier.com/retrieve/pii/S2211124722009822
Type of resourceText
Access conditionsOpen access
Terms of useLicence Attribution 4.0 International (CC BY 4.0) icon
Public note© 2022 The Author(s). This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). Published 2 August 2022.
Created on2024-08-22 09:17:12