Utjecaj propofola i N-acetilcisteina na razinu oksidacijskog stresa na psu kao modelu

doctoral thesis

• Title (english): Effects of propofol and N-acetylcystein on oxidative stress in a dog model
• Author: Robert Baronica
• Mentor: Goran Bačić (mentor); MBZ: 199334
• Mentor: DINKO TONKOVIĆ (sumentor)
• Committee member: Zoran Tadić (član povjerenstva)
• Committee member: Ana Cipak Gaparovic (član povjerenstva)
• Committee member: Tugomir Karadjole (član povjerenstva); MBZ: 233666
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2014, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 577 - Biochemistry. Molecular biology. Biophysics

Abstract

Cilj rada bio je usporediti utjecaj anestezije izofluranom ili propofolom uz dodatak N-acetilcistena kod laparotomijske i laparoskopske ovarijektomije kuja na koncentracije malondialdehida, endogenih peroksida i ukupnih antioksidansa. Ispitivano je i postoji li sinergističko djelovanje propofola i N-acetilcisteina. Provedeno je prospektivno, randomizirano, kontrolirano istraživanje koje je uključilo 61 kuju dobrog općeg stanja. Ispitivane životinje podijeljene su u 6 skupina prema kirurškoj tehnici i vrsti anestezije. Uzorci krvi za analizu uzeti su nakon uvoda u anesteziju, na kraju operacijskog zahvata i 24 sata nakon zahvata. Analizirani su metodama tekućinske kromatografije visoke djelotvornosti i kolorimetrijskim, enzimatskim testovima. Višestrukim usporedbama skupina uglavnom nisu nađene statistički značajne razlike u pokazateljima oksidacijskog stresa. Iako dobiveni rezultati nisu potvrdili prvobitnu hipotezu, analizom statističkih razlika koje su pronađene kao i vizualnom inspekcijom grafičkih prikaza zapažen je trend koji ukazuje da bi laparoskopski učinjena ovarijektomija i anestezija propofolom uz dodatak N-acetilcisteina mogle imati povoljne učinke na smanjenje oksidacijskog stresa.

Abstract (english)

The objectives of this study were to investigate the effects of anesthesia with isoflurane or propofol with addition of N-acetylcysteine during open and laparoscopic ovariectomy in bitches on malondialdehyde concentration, total oxidative status and antioxidant capacity. Also, the aim was to investigate possible synergistic effects of propofol and N-acetylcysteine. The study was prospective, randomised and controlled. 61 female dogs were allocated in 6 groups according to surgical technic and type of anesthesia. Blood samples for the analysis were taken after the induction of anesthesia, at the end of the procedure and 24 hours after. High performance liquid chromatography and colorimetric, enzymatic tests were used for the analysis. In most of the groups statistically significant differences in parameters of oxidative stress were not found. However, analysis of statistical differences which were found and observation of positive trends during perioperative period, discerned trend that laparoscopic surgery and anestehsia with propofol could have beneficial effects.

• Keywords: oksidacijski stres; propofol; N-acetilcistein; ovarijektomija
• Keywords (english): oxidative stress; propofol; N-acetylcystein; ovariectomy
• Language: croatian
• URN:NBN: urn:nbn:hr:217:001478
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-05-17 12:07:15