Izolacija i karakterizacija mikrosatelitnih biljega ljekovite kadulje (Salvia officinalis L., Lamiaceae)

doctoral thesis

• Title (english): Isolation and characterization of microsatellite markers in common sage (Salvia officinalis L., Lamiaceae)
• Author: Ivan Radosavljević
• Mentor: Zlatko Liber (mentor)
• Committee member: Zlatko Šatović (član povjerenstva)
• Committee member: Zlatko Liber (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2012, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology
• Universal decimal classification (UDC): 57 - Biological sciences. Physical anthropology. Biodiversity

Abstract

Cilj ove disertacije bio je razvijanje i primjena mikrosatelitnih biljega na populacijama ljekovite kadulje (Salvia officinalis L.). Isto tako nastojalo se primijeniti razvijene biljege na drugim vrstama roda Salvia L. te vrsti Rosmarinus officinalis L.. Razvijeno je ukupno 29 biljega, a primjenom osam najinformativnijih provedena je populacijsko-genetička analiza 45 populacija ljekovite kadulje s Balkanskog poluotoka. Srodstveni odnosi između većine populacija bili su korelirani s njihovom geografskom udaljenošću. Izuzetak je pet populacija za koje se pretpostavlja da nisu prirodne nego potječu iz uzgoja. Prema alelnom bogatstvu središte genetičke raznolikosti ljekovite kadulje nalazi se u Srednjoj i Južnoj Dalmaciji. Primjena mikrosatelitnih biljega ljekovite kadulje na kratkozupčastoj kadulji (S. brachyodon Vandas), otkrila je prolaz populacije s poluotoka Pelješca kroz genetsko usko grlo. Dobiveni rezultat naglašava potrebu preispitivanja statusa ugroženosti ove endemične vrste. Mikrosatelitna analiza ljekovite i grčke kadulje na otoku Visu (S. fruticosa Mill.) potvrdila je postojanje hibridne svojte S x auriculata Mill..

Abstract (english)

The goal of this dissertation was development and application of microsatellite markers on common sage populations, as well as their application on other sage species and Rosmarinus officinalis L. Overall, 29 markers were developed, and by using eight of highest informativity, population genetic analysis of 45 common sage populations from Balkan peninsula was performed. Relationships between most of populations were corelated with their geographical distribution, with exeption of 5 populations which are believed to originate from cultivation. According to allelic richness, center of common sage genetic diversity is located in Central and Southern Dalmatia. Apllication of microsatellite markers from common sage on Salvia brachyodon Vandas revealed a recent bottleneck event in population from Pelješac peninsula, thus stressing the need for redefining of conservation status of this endemic species. Microsatellite analysis of common and greek sage (S. fruticosa Mill.) from Island of Vis confirmed presence of lineage of hybrid origin, S x auriculata Mill..

• Keywords: mikrosateliti; ljekovita kadulja; cross-amlifikacija; populacijska genetika
• Keywords (english): mikrosatellites; common sage; cross-amplification; population genetics
• Language: croatian
• URN:NBN: urn:nbn:hr:217:897040
• Study programme: Title: Biology; Study programme type: university; Study level: postgraduate; Academic / professional title: doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija (doktor/doktorica znanosti, područje prirodnih znanosti, polje biologija)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2019-08-15
• Created on: 2017-06-08 10:11:14