Upotreba digitalnih mikroskopskih slika u histološkim i histokemijskim metodama

undergraduate thesis

• Title (english): The use of digital microscopic pictures in histological and histochemical methods
• Author: Ana Bekavac
• Mentor: Romana Gračan (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2017-07-14, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Histologija je grana biologije koja se bavi proučavanjem tkiva, a histokemija je svojevrsna poveznica biokemije i histologije. Različitim histološkim i histokemijskim metodama mogu se adekvatno obojati tkiva pri čemu se olakšava njihova vizualizacija. Tkivo prolazi pripremu koja podrazumijeva fiksaciju, dehidriranje, prosvjetljivanje, uklapanje u parafin i parafinske blokove, rezanje te naposljetku bojanje ili označavanje. Za bojanje u histologiji se najčešće koristi kombinacija boja hematoksilina i eozina. Označavanje molekula histokemijskim metodama može podrazumijevati imunofluorescenciju i hibridizaciju in situ. U moderno doba, dolazi do digitalizacije mikroskopskih slika. Digitalizirane mikroskopske slike su korak naprijed u laboratorijskoj dijagnostici jer pružaju objektivnost i kvantifikaciju struktura na samim slikama te veću objektivnost i točnost u analizi. Time je došlo do procvata takozvane histoinformatike koja se iz dana u dan sve više unaprjeđuje.

Abstract (english)

Histology is a branch of biology, which studies tissues and histochemistry is a link between biochemistry and histology. Different types of histological and histochemical methods are used for adequate tissue staining. Staining ensures better visualisation of the tissue. Tissues must be prepared in a specific protocol. The protocol starts with fixation, next is dehydration, clearing, embedding in paraffin blocks and the last is staining or marking. In histology, the most common staining method is haematoxylin and eosin staining. Histochemical methods which are used for identification of chemical compounds are immunofluorescence and in situ hybridisation. In the modern age, digitalisation of microscopic pictures happened. Digital microscopic pictures represent a step forward in laboratory diagnostics because they are unbiased and they offer higher accuracy in analysis. All of that conditioned the development of so called histoinformatics which is improving day by day.

• Keywords: histologija; histokemija; tkivo
• Keywords (english): histology; histochemistry; tissue
• Language: croatian
• URN:NBN: urn:nbn:hr:217:265485
• Study programme: Title: Ecology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-09-18 07:24:11