Molekularna filogenetika pauka roda Nesticus (Arachnida, Araneae, Nesticidae)

undergraduate thesis

• Title (english): Molecular phylogenetics of spiders from the genus Nesticus (Arachnida, Araneae, Nesticidae)
• Author: Domagoj Gajski
• Mentor: Damjan Franjević (mentor)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2016, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Molekularna filogenetika je grana filogenije koja analizira srodstvene razlike na molekularnoj razini, uglavnom se obazirući na DNA i RNA sekvence, kako bi dobila informacije o evolucijskom odnosu organizama i njihovoj evolucijskoj povijesti. Svrha molekularne filogenije je izraditi filogenetičko stablo od prikupljenog skupa podataka preko sekvenci gena poznatih kao molekularnih biljega. Cilj ovog rada je opisati primjerom kako teče proces molekularno filogenetskog istraživanja. U ovom radu je proces opisan primjerom dokazivanja nove vrste špiljskog pauka Nesticus baeticus López-Pancorbo & Ribera, 2011 i njegovog uspoređivanja po filogeniji sa ostalim vrstama roda Nesticus koje su endemične za prostor Pirinejskog poluotoka kako bi ustvrdili njihovu srodnost. Za izradu stabla korištena je metoda najveće štedljivosti (Maximum parsimony) gdje su koristili vrstu iz roda Nesticus Thorell, 1869 sa područja Kine kao vanjsku grupu te je bootstrap-testom provjerena pouzdanost svakog čvora. Kod analize gotovog stabla došlo se do zaključka da vrste roda Nesticus sa Pirinejskog poluotoka nisu unutar jedne monofiletičke grupe te se pretpostavlja da su nezavisno tri puta kolonizirali Pirinejski poluotok. Kako bi se pretpostavka potvrdila ili odbacila, treba se napraviti molekularno filogenetska analiza svih mediteranskih pauka iz porodice Nesticidae za bolji prikaz evolucijskih događaja i kako bi se testirao molofiletički status trenutnog roda.

Abstract (english)

Molecular phylogenetics is a branch of phylogenetic analyzes of kinship differences at the molecular level, largely considering the DNA and RNA sequences, in order to obtain information about the evolutionary relationship of organisms and their evolutionary history. The purpose of the molecular phylogeny is to draw design a phylogenetic tree of the collected data set of sequences of genes known as molecular markers. The objective of this paper is to describe an example of the procedure of a study in molecular phylogenetics. In this paper, the procedure is described by how to prove a new species of cave spiders - Nesticus baeticus López-Pancorbo & Ribera, 2011 and its comparison in phylogeny with the other species of the genus Nesticus that are endemic to the area of the Iberian Peninsula. To create a tree they used the method of Maximum parsimony where they used a species form the genus Nesticus which is located in China as an outgroup and with the bootstrap test they checked the reliability of each node. In the final analysis of the tree, they came to the conclusion that all the species from genus Nesticus located on the Iberian Peninsula are not within the same monophyletic group and it is assumed that they colonized the Iberian Peninsula independently three times. To confirm or reject the hypothesis, the future research should be focused on resolving the philogeny of the Mediterranean Nesticidae and will include most of the Mediterranean species to test the molophyletic status of current genera.

• Keywords: molekularna filogenetika; Nesticus; pauci
• Keywords (english): molecular phylogenetics; Nesticus; spiders
• Language: croatian
• URN:NBN: urn:nbn:hr:217:943600
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: undergraduate; Academic / professional title: sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije (sveučilišni/a prvostupnik/prvostupnica (baccalaureus/baccalaurea) molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-01-02 09:48:42