Uzgojne značajke proteolitičkih sulfid-producirajućih bakterija na novom tipu hranjive podloge

master's thesis

• Title (english): Plate method for counting proteolytic sulphide-producing bacteria
• Author: Vladimir Ramljak
• Mentor: Jasna Hrenović (mentor)
• Committee member: Jasna Hrenović (predsjednik povjerenstva)
• Committee member: Ivančica Ternjej (član povjerenstva)
• Committee member: Zdravko Dolenec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2009, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Proteolitičke sulfid-producirajuće bakterije (PSPB) široko su rasprostranjene u vodi i sedimentu i predstavljaju vrlo dobar pokazatelj ekološkog stanja ekosistema. S ekološkog gledišta važno je razlikovati fiziološku skupinu PSPB-a od ostalih skupina bakterija koje proizvode H2S iz sulfata i tiosulfata. S tim ciljem razvijen je i iskušan novi tip podloge, pepton-cistein-amonijak-željezo citratni agar (PCA). Na tom je tipu podloge redovito dolazilo do stvaranja većeg broja PSPB bakterijskih kolonija nego na kontrolnoj podlozi (SIM). Za brojanje PSPB bakterijskih kolonija nastalih iz okolišnih uzoraka preporuča se simultana inkubacija istih uzoraka u aerobnim i anaerobnim uvjetima te bi za tumačenje rezultata trebalo uzeti veći broj nastalih kolonija.

Abstract (english)

The proteolytic sulphide-producing bacteria (PSPB) are widely distributed in the water and sediment and are a good indicator of the ecological status of ecosystems. From the ecological point of view it is important to distinguish the physiological group of PSPB from other bacteria producing H2S form sulphate or thiosulphate. A new medium named peptone-cysteine-ammonium-iron citrate agar (PCA) was developed and tested. The medium regularly gave higher CFU of PSPB than the used control medium (SIM). For the enumeration of PSPB from environmental samples, simultaneous incubation of the same samples in aerobic and anaerobic conditions is recommended and a higher number should be taken for interpretation.

• Keywords: proteolitičke sulfid-producirajuće bakterije; proizvodnja H2S; voda; sediment
• Keywords (english): plate count; proteins; putrefaction; sediment; sulphide-producing bacteria; water
• Language: croatian
• URN:NBN: urn:nbn:hr:217:699415
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-16 10:54:10