Koncentracija ukupnih koliforma u površinskim vodama na području grada Zagreba

master's thesis

• Title (english): Concentration of total coliform bacteria from surface water in area of city of Zagreb
• Author: Ivana Vešligaj
• Mentor: Jasna Hrenović (mentor)
• Committee member: Jasna Hrenović (predsjednik povjerenstva)
• Committee member: Mladen Kerovec (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Committee member: Davor Kovačević (član povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2009, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Istraživanje koncentracije ukupnih koliforma u površinskim vodama na području grada Zagreba provedeno je na uzorku čiste kulture vrste Escherichia coli i 19 uzoraka vode prikupljenih iz jezera Botaničkog vrta, 5 maksimirskih jezera te 7 potoka grada Zagreba. Za kultivaciju ukupnih koliforma korištene su četiri hranjive podloge: EC X Gluc agar, m-Faecal Coliform agar, Endo agar i MacConkey agar. Uzorci su nacijepljeni na pojedine medije metodom širenja razmaza. Određeni broj ukupnih koliforma i postotni udio E. coli u populaciji ukupnih koliforma u istom uzorku bio je značajno različit na različitim hranjivim podlogama. Uspoređujući podatke dobivene brojanjem izraslih kolonija koliformnih bakterija, ustanovljeno je da se koncentracije ukupnih koliforma bitno razlikuju s obzirom na lokaciju uzimanja uzoraka. Njihova najveća koncentracija utvrđena je u vodama tekućicama, što prvenstveno uključuje Vugrov potok, Vrapčak, Črnomerec i Srednjak. Niže koncentracije ukupnih koliforma utvrđene su u jezeru Botaničkog vrta, Petom maksimirskom jezeru , te u maksimirskim potocima Bliznec i Maksimirec. Na osnovu broja ukupnih koliforma, utvrđena je i kakvoća vode prema Uredbi o klasifikaciji voda Republike Hrvatske (˝Narodne novine˝ 77/98). U 6 od 19 ispitivanih uzoraka vode određena je V vrsta vode, dok su ostalu uzorci pokazali IV vrstu voda.

Abstract (english)

Concentration of total coliform bacteria from surface water in area of city of Zagreb, was investigated on sample of clean culture of Escherichia coli and 19 samples of water, collected at the lake of Botanical Garden, five lakes of Maksimir and at seven brooks of Zagreb. Four commercial solid media were used for cultivation of total coliform bacteria, and they are: ECX Gluc agar, Endo agar, m-Faecal Coliform agar and MacConkey agar. Samples was inoculated on each media with Plate Strake Method. The referred number of total coliform bacteria and the percentage of E.coli in the population of total coliform in the same sample was significantly different on various media. Comparing the dana optained from counting down overgrown colonies of coliform bacteria, was established that the concentratoin of total coliform bacteria differ according to the sample of the primary location. Their biggest concentration was determined in nonstagnant waters, which primarily includes brook of Vugrov, Vrapčak, Črnomerec and Srednjak. Lower concentration of total coliform bacteria was determined in the lake of Botanic garden, the Fifth lake of Maksimir and in the brooks of Maksimir, Bliznec and Maksimirec. The water quality was ratified on the base of the number of total coliform bacteria and the Water classification regulation of the Republic of Croatia (˝Narodne novine˝ 77/98). The V class of water was defined in 6 out of 19 analysed samples, whereas other samples showed the IV class of water.

• Keywords: ukupne koliformne bakterije; hranjive podloge; površinske vode
• Keywords (english): total coliform bacteria; solid media; surface media
• Language: croatian
• URN:NBN: urn:nbn:hr:217:327278
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-03-19 09:22:59