Kristalizacija derivata inzulina uz dodatak halogenida ; Integrirani pristup poučavanju probavnih ezima

master's thesis

• Title (english): Crystallization of insulin derivatives with addition of halides
• Author: Adela Jurković
• Mentor: Dubravka Matković-Čalogović (mentor); MBZ: 108862
• Mentor: Draginja Mrvoš-Sermek (mentor)
• Committee member: Dubravka Matković-Čalogović (predsjednik povjerenstva); MBZ: 108862
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

Ovaj diplomski rad sastoji se od istraživačkog i metodičkog dijela. U istraživačkom dijelu rada opisana je
kristalizacija halogenidnih i nehalogenidnih derivata goveđeg i ljudskog inzulina primjenom metode difuzije
para viseće kapi. Korištene su Hampton Research VDX ploče od 24 spremnika. Ploče su držane u mraku na
temperaturi od 20 °C. Varijacijom koncentracije precipitantnih reagensa, koncentracije proteina i veličine kapi
dobiveni su monokristali derivata inzulina. Dodatkom natrijevog klorida i soli odgovarajućeg metala
kristaliziraju sljedeći derivati inzulina: cinkov(II) klor-derivat goveđeg i ljudskog inzulina; kobaltov(II) klor-,
niklov(II) klor-, manganov(II) klor-, bakrov(II) klor- i molibdenov(VI) klor-derivat ljudskog inzulina. Uz
dodatak natrijevog jodida kristalizira cinkov(II) jod-derivat ljudskog inzulina, a uz dodatak soli odgovarajućeg
metala kristaliziraju vanadijev(IV) i molibdenov(VI) derivat ljudskog inzulina. Strukture cinkovog(II) klor-,
cinkovog(II) jod- i kobaltovog(II) klor-derivata ljudskog inzulina određene su rentgenskom strukturnom
analizom, čime se dobio uvid u koordinaciju metalnih iona u inzulinu kao i u konformaciju molekule inzulina.
Navedeni derivati kristaliziraju u trigonskom sustavu, u prostornoj grupi R3. Cinkov(II) klor-derivat pripada 4Zn
tipu (T3R3
f tipu). Cinkov(II) jod-derivat pripada 2Zn tipu, ali i T3R3 tipu koji je inače atipičan za 2Zn tip.
Kobaltov(II) klor-derivat ljudskog inzulina pripada 2Zn tipu (T6 tipu).
U metodičkom dijelu rada pod naslovom Integrirani pristup poučavanju probavnih enzima, predložen
je nastavni materijal za integrirani način izvođenja nastavne jedinice „Probavni enzimi“. Načinjen je pregled
nastavnih programa iz kemije i biologije za osnovnu i srednju školu s osvrtom na probavne enzime i obrazovne
ishode koji se očekuju za pojedino obrazovno razdoblje. Priložena je uputa za nastavnike, prijedlozi pokusa i
testovi za samoocjenjivanje (grupe A i B) te zadaci za provjeru znanja. Nastavni materijal je prilagođen
obrazovnom razdoblju četvrtog razreda gimnazije u završnom programskom dijelu.

Abstract (english)

This thesis consists of two parts: the research and methodological one. In the research part of the thesis
crystallization of halogen and non-halogen derivatives of bovine and human insulin by the hanging drop vapour
diffusion method is described. Hampton Research VDX plates consisting of 24 wells were used. The plates were
kept in the dark at 20 oC. Single crystals of insulin derivatives were obtained by variations in the concentration
of the precipitant solution, protein concentration and drop size. The following insulin chloro derivatives were
crystallized with addition of NaCl and a metal salt in the precipitant solution: zinc(II) chloro-derivative of bovine
and human insulin; cobalt(II) chloro-, nickel(II) chloro-, manganese(II) chloro-, copper(II) chloro- and
molybdenum(VI) chloro-derivatives of human insulin. By addition of NaI the zinc(II) iodo derivative of human
insulin was crystallized. By addition of a metal salt vanadium(IV) and molybdenum(VI) derivatives of human
insulin were crystallized. The X-ray diffraction experiment was conducted on the zinc(II) chloro, zinc(II) iodo
and cobalt(II) chloro derivatives of human insulin to determine their molecular and crystal structure. The three
mentioned derivatives were found to crystallize in the trigonal system, R3 space group. The zinc(II) chloroderivative
exhibits the 4Zn form (T3R3
f form). The zinc(II) iodo-derivative is of the 2Zn type, but also in the
T3R3 form, usually atypical for the 2Zn type; and the cobalt(II) chloro-derivative exhibits the 2Zn type (T6 form).
In the methodological part of the Thesis entitled Integrated approach to teaching digestive enzymes,
teaching material for the integrated lesson unit “Digestive enzymes” is suggested. An overview of the teaching
curriculum in chemistry and biology for elementary and high school, with the reference to digestive enzymes,
was made. Instructions for teachers, tests for self-evaluation (groups A and B) with experiments and a
knowledge assessment test for fourth grade high school students are enclosed.

• Keywords: derivati inzulina; kristalizacija; metoda difuzije para viseće kapi; integrirano poučavanje; poučavanje kemije; probavni enzimi
• Keywords (english): crystallization; derivatives of insulin; vapour diffusion method; digestive enzymes; integrated teaching; teaching chemistry
• Language: croatian
• URN:NBN: urn:nbn:hr:217:751123
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-07-09 13:29:51