Bioremedijacija kationskog surfaktanta benzalkonijevog klorida

master's thesis

• Title (english): Bioremediation of kationic surfactant benzalkonium chloride
• Author: Domagoj Đapić
• Mentor: Tomislav Ivanković (mentor)
• Committee member: Tomislav Ivanković (predsjednik povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Committee member: Dubravka Hranilović (član povjerenstva)
• Committee member: Ivančica Ternjej (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018-07-02, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Tema je mojega diplomskog rada bioremedijacija benzalkonijeva klorida, cikličnog aromatskog ugljikovodika koji služi kao biocid i kationski surfaktant. Upotrebljava se u prašcima za pranje rublja i omekšivačima za tkanine. Bioremedijacija je svaki proces koji se koristi mikroorganizmima u svrhu vraćanja okoliša u prirodno stanje prije nego što je nastupilo onečišćenje ili zagađenje iz nekog antropogenog izvora. Cilj mojega diplomskog rada jest utvrditi mogu li benzalkonijev klorid razgrađivati prirodno prisutne bakterije iz Jarunskog jezera i jezera u Botaničkom vrtu ili bakterije iz aktivnog mulja, otpadne vode, tla te suspenzije bakterija Pseudomonas fluorescens i Pseudomonas aeruginosa. Nije bilo na pločama s bakterijskim agarom kolonija bakterija P. aeruginosa i P. fluorescens, što znači da te bakterije nisu razgrađivale benzalkonijev klorid. Bakterije iz jezera Jarun i jezera u Botaničkom vrtu nisu razgradile benzalkonijev klorid. Bakterije iz otpadne vode, aktivnog mulja i tla koje smo izolirali u laboratoriju razgradile su benzalkonijev klorid u potpunosti. Bakterije koje su identificirane kao razgrađivači benzalkonijevog klorida jesu Agrobacterium radiobacter, Stenotrophomonas maltophilia i Bacillus cereus.

Abstract (english)

The topic of my graduate thesis is bioremediation of benzalkonium chloride, a cyclic aromatic hydrocarbon that serves as a biocide and a cationic surfactant. It is used in laundry detergents and fabric softeners. Bioremediation is any process that uses microorganisms for the purpose of restoring the environment to a natural state before pollution or contamination from an anthropogenic source occured. The aim of my graduate thesis is to determine whether benzalkonium chloride can be degraded by native bacteria from Lake Jarun and lake in botanical garden or specifically grown bacteria from activated sludge, wastewater, soils and suspensions of Pseudomonas fluorescens and Pseudomonas aeruginosa. It was not on bacterial agar plates any bacterial colonies of P. aeruginosa and P. fluorescens, meaning that these bacteria did not break down benzalkonium chloride. Native bacteria from Jarun lake and lake in the botanical garden did not degrade benzalkonium chloride either. Bacteria from the waste water, the activated sludge and the soil we isolated in the laboratory degraded the benzalkonium chloride completely. Bacteria identified as benzalkonium chloride degraders were Agrobacterium radiobacter, Stenotrophomonas maltophilia and Bacillus cereus.

• Keywords: bioremedijacija; benzalkonijev klorid; Agrobacterium radiobacter
• Keywords (english): bioremediation; benzalkonium chloride; Agrobacterium radiobacter
• Language: croatian
• URN:NBN: urn:nbn:hr:217:761672
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2018-08-28 11:30:02