Morfološke značajke potočnog raka (Austropotamobius torrentium (Schrank, 1803))

master's thesis

• Title (english): Morphological characteristics of stone crayfish (Austropotamobius torrentium (Schrank, 1803))
• Author: Vjera Pavić
• Mentor: Ivana Maguire (mentor)
• Committee member: Ivana Maguire (predsjednik povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Tomislav Ivanković (član povjerenstva)
• Committee member: Jasna Lajtner (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2018-07-03, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Austropotamobius torrentium jedna je od četiri nativne europske vrste porodice Astacidae hrvatskih slatkovodnih ekosustava. Nedavna molekularno-filogenetska istraţivanja vrste otkrila su sedam značajno divergentnih monofiletskih filogrupa. Morfološke značajke vrste nastaju i mijenjaju se pod utjecajem genetičkih faktora kao i adaptacijama na specifične stanišne i ekološke uvjete. Sukladno tome, glavni cilj ovog rada je proučiti koje morfološke karakteristike jasno razdvajaju populacije rakova, sakupljene na 15 lokaliteta, prethodno grupiranih u filogrupe. Stoga, 245 jedinki rakova, unutar pet filogrupa, analizirane su tradicionalnim i geometrijskim morfometrijskim metodama. Tradicionalna diskriminantna analiza uključivala je 24 morfometrijske mjere po raku, dok je geometrijska morfometrija uključivala analizu 22 specifične točke na leĎnoj strani glavopršnjaka. UtvrĎene su statistički značajne razlike u većini mjerenih morfometrijskih karakteristika meĎu istraţivanim populacijama muţjaka i ţenki. Time su potvrĎena molekularno-filogenetska istraţivanja o postojanju intraspecijske divergencije meĎu različitim filogrupama ove vrste rakova. Ovim radom potvrĎeno je da su obje morfometrijske analize kombinirane sa statističkim metodama korisne u razlikovanju i razdvajanju filogrupa rakova. Dobiveni rezultati posluţit će u svim daljnjim istraţivanjima ove vrste, kako na području Hrvatske, tako i na području Europe.

Abstract (english)

Austropotamobius torrentium is one of four native European crayfish species inhabiting Croatian freshwater ecosystems. Recent molecular-phylogenetic studies of A. torrentium discovered existence of seven divergent monophyletic phylogroups within the species. The differences in morphometric characteristics among populations of stone crayfish are most likely the result of the combination of genetic and environmental mechanisms. The main goal of this graduate thesis was to test if some morphological characteristics clearly separate stone crayfish, among 15 populations, belonging to different, previously identified, phylogroups. For this purpose 245 stone crayfish, belonging to five different phylogroups, were analysed through traditional and, for the first time, geometric morphometric analyses. Traditional multivariate discriminant analyses included 24 morphometric features per crayfish, while geometric morphometrics comprised analyses of 22 landmarks on the dorsal side of cephalothorax. It has been established that there are statistically significant differences among the populations of males and females in measured morphometric characteristics. These results confirmed the molecular-phylogenetic studies, this species shows intraspecific divergence among different phylogroups. This thesis has confirmed that both morphometric analyses, combined with statistical methods, are useful in differentiating and separating crayfish populations. The obtained results will be useful in all further research of A. torrentium, both in Croatia and in Europe.

• Keywords: Austropotamobius torrentium; Astacidae; tradicionalna morfometrija; geometrijska morfometrija; filogrupe
• Keywords (english): Austropotamobius torrentium; Astacidae; traditional morphometry; geometric morphometry; phylogroups.
• Language: croatian
• URN:NBN: urn:nbn:hr:217:874522
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2020-08-29
• Created on: 2018-08-29 08:28:29