Imunohistokemijska analiza transkripcijskog faktora SOX2 tijekom fetalnog razvoja čeonog režnja mozga čovjeka

master's thesis

• Title (english): Immunohistochemical analysis of SOX2 transcription factor in the human frontal lobe during fetal brain development
• Author: Janja Kopić
• Mentor: Željka Krsnik (mentor)
• Mentor: Domagoj Đikić (mentor)
• Committee member: Domagoj Đikić (predsjednik povjerenstva)
• Committee member: Biljana Balen (član povjerenstva)
• Committee member: Renata Šoštarić (član povjerenstva)
• Committee member: Renata Matoničkin Kepčija (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2019-02-15, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Čeoni režanj mozga čovjeka odgovoran je za kompleksne kognitivne funkcije, razmišljanje, donošenje odluka, govor, voljne pokrete i dr. Kora velikog mozga rezultat je procesa reguliranih diferencijalnom ekspresijom gena, poput proliferacije, migracije, diferencijacije i molekularne specifikacije. Tijekom fetalnog razvoja, moždana kora dobiva laminarno ustrojstvo formiranjem privremenih fetalnih zona: ventrikularna, subventrikularna, intermedijarna, subplate zona, kortikalna ploča i marginalna zona. Neuroni nastaju tijekom embrionalnog razvoja od neuralnih matičnih stanica. Gen SOX2 bitan je za održavanje multipotentnosti matičnih stanica te usmjeravanje njihove neuralne diferencijacije. Unatoč tome, ekspresijski obrazac SOX2 u moždanoj kori čeonog režnja nije poznat. U ovom radu su korišteni uzorci postmortalnih tkiva mozga čovjeka te su pomoću histološke metode bojanja po Nisslu prikazane njihove privremene fetalne zone a pomoću imunohistokemije određena ekspresija i distribucija SOX2 u fetalnim zonama tijekom ranog i srednjeg fetalnog razdoblja. Metodom dvostrukog imunohistokemijskog bojanja nije zamijećena kolokalizacija SOX2 i NeuN, već je pokazano da pojedine SOX2 imunopozitivne stanice kolokaliziraju sa GFAP. Najveća ekspresija SOX2 je bila zabilježena u glavnim proliferativnim zonama: ventrikularnoj i subventrikularnoj zoni. Međutim uočen je pomak u distribuciji SOX2 tijekom kasnog srednje fetalnog razdoblja u subplate zonu i kortikalnu ploču. Time se otvaraju pitanja o novim potencijalnim ulogama SOX2 gena tijekom kasnog srednje fetalnog razdoblja.

Abstract (english)

Frontal lobe of the human brain is responsible for complex cognitive functions, thinking, decision-making, speech, voluntary movements, and others. Cerebral cortex is result of processes regulated by differential gene expression, such as proliferation, migration, differentiation and molecular specifications. During fetal development, cerebral cortex gets its laminar structure by the formation of transient fetal zones: ventricular, subventricular, intermediate, subplate zone, cortical plate and marginal zone. Neurons arise during embryonic development from neural stem cells. Gene SOX2 is essential for maintaining multipotent stem cell features and regulates neuronal differentiation. However, its expression pattern in human frontal cortex remains unknown. In this thesis, samples of postmortem human brain tissue were stained with histological method by Nissl to visualize their transient fetal zones. Moreover, immunohistochemistry showed distribution of SOX2 in transient fetal zones during early and mid-term fetal periods. Double-immunohistochemistry didn't show SOX2 and NeuN colocalization, but revealed colocalization of SOX2 and GFAP. Highest expression levels of SOX2 were observed in proliferative zones: ventricular and subventricular zone. However, a shift in SOX2 distribution was noted during the late mid-fetal period in the subplate zone and the cortical plate. This raises questions about new potential roles of SOX2 in late mid-fetal period.

• Keywords: neurogeneza; čeoni režanj; fetalne zone; SOX2; neuralne matične stanice
• Keywords (english): neurogenesis; frontal lobe; fetal zones; SOX2; neural stem cells
• Language: croatian
• URN:NBN: urn:nbn:hr:217:360208
• Study programme: Title: Molecular Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra molekularne biologije (magistar/magistra molekularne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access; Embargo expiration date: 2021-02-15
• Created on: 2019-03-26 09:35:06