Učinak naringenina na simbiozu hidre i alge te mogućnost primjene predloženog pokusa u školi

master's thesis

• Title (english): The effect of naringenin on hydra-alga symbiosis and possibility of implementation of the given experiment in school
• Author: Valentina Bartol
• Mentor: Goran Kovačević (mentor)
• Mentor: Damir Sirovina (mentor)
• Committee member: Goran Kovačević (predsjednik povjerenstva)
• Committee member: Damir Sirovina (član povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2017-02-23, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Flavonoidi su biljni pigmenti koji su pronađeni u voću, povrću, čaju, cvijeću i vinu. Sve su više zastupljeni u svakodnevnom životu te je od važnosti i značaja istraživati njihovu ulogu za žive organizme. Jedan od flavonoida je i naringenin koji pripada skupini flavonona. Njegov učinak je praćen na simbiozu hidre i alge. U ovom radu kao test organizmi korišteni su: Hydra viridissima Pallas, 1766 i Hydra oligactis Pallas, 1766 te endosimbiotske alge izolirane iz domaćina zelene hidre Mychonastes homosphaera (Skuja) Kalina i Punčochářová, Desmodesmus subspicatus (Chodat) Hegewald i Schmidt i slobodnoživuća srodna vrsta Chlorella vulgaris Beij. Napravljena je morfološka i citološko – histološka analiza pri koncentracijama 0,2 gL−1, 0,25 gL−1 i 0,3 gL−1 naringenina te je utvrđena koncentracija od 0,25 gL−1 naringenina pri kojoj zadani uvjeti posebice ne pogoduju rastu organizama. Praćena je smrtnost, pupanje, izlučivanje sluzi, migracija te podražljivost hidri, kao i vijabilnost algi. Zbog mogućnosti mijenjanja i prilagođavanja pokusa različitim vremenskim i prostornim uvjetima te dostupnosti potrebnog materijala i pribora, predloženo je njegovo izvođenje u školi u sklopu izvannastavnih aktivnosti ili školskog projekta. Kako bi se istražilo imaju li nastavnici odgovarajuće uvjete za takav način rada, nastavnicima iz područja biologije i kemije u različitim školama i županijama dodijeljena je anonimna anketa. Utvrđeno je da škole uglavnom nude izvannastavne aktivnosti i da imaju odgovarajući pribor za izvedbu predloženog pokusa.

Abstract (english)

Flavonoids are plant pigments found in fruits, vegetables, tea, flowers and wine. They are even more present in everyday life and it is of importance and significance to investigate their roleup on organisms. Naringenin is a flavonoid that belongs to the group of flavanones. Its effect was monitored on a hyda – algasymbiosis. As test organisms were used: Hydra viridissima Pallas, 1766 and Hydra oligactis Pallas, 1766 as well as endosymbiotic algae isolated from the hydra host Mychonastes homosphaera (Skuja) Kalina and Punčochářová, Desmodesmus subspicatus (Chodat) Hegewald and Schmidtand, related free – living species Chlorella vulgaris Beij. Morphological and cyto – hystological analysis was made using concentrations of 0,2 gL−1, 0,25gL−1 and 0,3 gL−1 of naringenin and it was determined that the concentration of 0,25 gL−1 does not favor the growth of organisms. Mortality, budding, mucus secretion, migration and contractions of hydra as well as viability of algal cells were monitored. Because of the possibility of changing and adapting the experiment in different temporal and spatial conditions and the availability of materials and accessories, its performance was suggested in school as a part of extra – curricular activities or school project. In order to investigate, if teachers would have proper pre - conditions for this kind of work, teachers of biology and chemistry in different schools and counties, were assigned an anonymous survey. Schools generally offer extra – curricular activities and they have appropriate tools to perform the proposed experiment.

• Keywords: flavonoidi; Hydra viridissima; Hydra oligactis; Chlorella; anketiranje
• Keywords (english): flavonoids; Hydra viridissima; Hydra oligactis; Chlorella; survey
• Language: croatian
• URN:NBN: urn:nbn:hr:217:005831
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-20 11:08:17