Udio fenolnih spojeva, antioksidacijska aktivnost i fotosintetska učinkovitost različitih dijelova vrčeva hibrida roda Sarracenia L.

master's thesis

• Title (english): Content of phenolic compounds, antioxidant activity and photosynthetic efficiency of different parts of pitchers of Sarracenia L. hybrids
• Author: Martina Tušek
• Mentor: Mirta Tkalec (mentor)
• Committee member: Mirta Tkalec (predsjednik povjerenstva)
• Committee member: Ines Radanović (član povjerenstva)
• Committee member: Draginja Mrvoš-Sermek (član povjerenstva)
• Committee member: Jasmina Rokov Plavec (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2015-09-15, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Sarracenia je rod mesojednih biljaka rasprostranjen u području Sjeverne Amerike, a karakteriziraju ga listovi preobraženi u vrčeve pomoću kojih mame, hvataju i probavljaju kukce. Cilj ovog istraživanja bio je utvrditi kako se morfološki različiti hibridi međusobno razlikuju u udjelu fotosintetskih pigmenata i fenolnih spojeva, fotosintetskoj učinkovitosti i antioksidacijskoj aktivnosti u različitim dijelovima vrčeva. Analizirana su četiri dijela vrča - poklopac, „krilo“, gornji dio vrča i donji dio vrča morfološki različitih hibrida (A, B i C) iz roda Sarracenia. Utvrđeno je smanjenje fotosintetske učinkovitosti u poklopcu i gornjem dijelu vrča u odnosu na donji dio vrča i „krilo“, osobito kod hibrida B te povećana stopa fotosinteze kod hibrida C. Hibrid B također se isticao većim udjelom klorofila i karotenoida u „krilu“ i donjem dijelu vrča u odnosu na ostala dva hibrida. Utvrđena je povezanost udjela fenolnih spojeva i fotosintetskih pigmenata s obojenošću dijelova vrča te sudjelovanjem u privlačenju i hvatanju kukaca. Viši udio fenolnih spojeva, osobito antocijana, izmjeren je u poklopcu i gornjem dijelu vrča koji su crveni te više služe karnivoriji dok su udio klorofila i fotosintetska učinkovitost bili viši u „krilu“ koje je zelene boje te sudjeluje u fotosintezi. Antioksidacijska aktivnost je bila visoka u svim dijelovima vrčeva svih hibrida.

Abstract (english)

Sarracenia is a genus of carnivorous plants widespread in North America and is characterized by leaves modified into pitchers which lore, hunt and digest insects. The aim of this study was to analyze the variance in photosynthetic pigments and phenolic compounds as well as photosynthetic efficiency and antioxidant activity in different pitcher parts of morphologically different hybrids. Four parts of the pitcher – operculum, “wing”, pitcher upper part and pitcher lower part were analyzed in morphologically different Sarracenia hybrids (A, B and C). The photosynthetic efficiency of operculum and pitcher upper part was lower than in pitcher lower part and “wing”, especially in hybrid B. However, in hybrid C the higher rate of photosynthesis was noticed. Hybrid B also has higher amount of chlorophyll and carotenoids in “wing” and pitcher lower part compared to the other two hybrids. Results indicate that the amount of phenolic compounds and photosynthetic pigments is related to coloration of pitcher parts, and therefore participating in attraction and trapping insects. The higher amount of phenolic compounds, especially anthocyanin, is measured in operculum and pitcher upper part, which are red-colored and involved more in carnivory, while amount of chlorophyll and photosynthetic efficiency were higher in “wing” that is green-colored and is involved more in photosynthesis. Antioxidant activity was high in all pitcher parts of all three hybrids.

• Keywords: mesojedne biljke; fotosinteza; fotosintetski pigmenti; Sarracenia; antocijani; flavonoidi; ukupni fenoli; obojenost vrčeva
• Keywords (english): carnivorous plants; photosynthesis; photosynthetic pigments; Sarracenia; anthocyanins; flavonoids; content of total phenols; pitcher coloration
• Language: croatian
• URN:NBN: urn:nbn:hr:217:414272
• Study programme: Title: Integrated undergraduate and graduate university study of Biology and Chemistry Education; specializations in: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Course: Integrated undergraduate and graduate university study of Biology and Chemistry Education; Study programme type: university; Study level: integrated undergraduate and graduate; Academic / professional title: magistar/magistra edukacije biologije i kemije (magistar/magistra edukacije biologije i kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2017-04-24 09:04:42