Isolation and structural elucidation of vernix caseosa TG-estolides with TLC, HPLC-ESI/MS and GC-MS

master's thesis

• Title (croatian): Izolacija i određivanje TG-estolida iz uzorka sirastog maza upotrebom TLC-a, HPLC-ESI/MS-a i GC-MS-a
• Author: Hrvoje Dumić
• Mentor: Jozef Cvačka https://orcid.org/0000-0002-3590-9009 (mentor)
• Committee member: Predrag Novak (predsjednik povjerenstva)
• Committee member: Željka Soldin (član povjerenstva)
• Committee member: Vesna Petrović Peroković (član povjerenstva)
• Committee member: Ivana Biljan (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Chemistry); Zagreb
• Defense date and country: 2020-01-28, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Chemistry

Abstract

A complete isolation of vernix caseosa triacylglycerol estolides with combination of thin-layer chromatography (TLC) and high-performance liquid chromatography (HPLC), and their partial structural elucidation with high-performance liquid chromatography – mass spectrometry (HPLC-MS) and gas chromatography – mass spectrometry (GC-MS) hyphenated systems was performed. New semi-preparative TLC method for isolation and semi-preparative HPLC method for additional purification of vernix caseosa triacylglycerol estolides were developed. Structural analysis of intact triacylglycerol estolides with LC-MS system revealed 126 structural isomers and after transesterification of triacylglycerol estolides, analysis of fatty acid methyl esters with GC-MS 71 different fatty acids that build vernix caseosa triacylglycerol estolides were observed.

Abstract (croatian)

Upotrebom tankoslojne kromatografije (TLC) i tekućinske kromatografije visoke djelotvornosti (HPLC) provedena je potpuna izolacija triacilglicerol estolida iz uzorka sirastog maza (vernix caseosa) te je djelomično određena njihova struktura upotrebom vezanih sustava tekućinska kromatografija visoke djelotvornosti – masena spektrometrija (HPLC-MS) i plinska kromatografija – masena spektrometrija (GC-MS). Razvijena je nova metoda semi-preparativne tankoslojne kromatografije za izolaciju i nova metoda semi-preparativne tekućinske kromatografije visoke djelotvornosti za dodatno pročišćavanje triacilglicerol estolida iz uzorka sirastog maza. Strukturna analiza triacilglicerol estolida, u njihovom intaktnom obliku, upotrebom vezanog sustava HPLC-MS otkrila je postojanje 126 strukturnih izomera. Također je, nakon transesterifikacije uzorka, strukturnom analizom metilnih estera masnih kiselina upotrebom vezanog sustava GC-MS otkriveno 71 različitih masnih kiselina koje grade triacilglicerol estolide u sirastom mazu.

• Keywords: GC; HPLC; MS; TLC; triacylglycerol estolides; vernix caseosa
• Keywords (english): GC; HPLC; MS; sirasti maz; TLC; triacilglicerol estolidi
• Language: english
• URN:NBN: urn:nbn:hr:217:643362
• Study programme: Title: Graduate university studies in chemistry; specializations in: Research programme; Course: Research programme; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra kemije (magistar/magistra kemije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Open access
• Created on: 2020-02-06 15:28:14