Raznolikost patogenih bakterija porodice Anaplasmataceae u pasa na području Republike Hrvatske

master's thesis

• Title (english): The diversity of patogenic bacteria within the Anaplasmataceae family in dogs from Croatia
• Author: Vanina Pogačar
• Mentor: Relja Beck (mentor)
• Mentor: Duje Lisičić (mentor)
• Committee member: Duje Lisičić (predsjednik povjerenstva)
• Committee member: Božena Mitić (član povjerenstva)
• Committee member: Jasna Hrenović (član povjerenstva)
• Committee member: Ana Galov (član povjerenstva)
• Granter: University of Zagreb; Faculty of Science; (Department of Biology); Zagreb
• Defense date and country: 2014-10-29, Croatia
• Scientific / art field, discipline and subdiscipline: NATURAL SCIENCES; Biology

Abstract

Porodica Anaplasmataceae dio je obitelji malih unutarstaničnih proteobakterija koja obuhvaća rodove Anaplasma, Ehrlichia, Neorickettsia, Wolbachia, Aegiptianella i Candidatus Neoehrlihia. Ove bakterije preživljavaju kao unutarstanični endosimbionti, no istovremeno mogu uzrokovati bolesti ljudi i životinja. Bakterije roda Anaplasma prenose se vektorima (krpelji šikare), a na području Europe najznačajniji je krpelj vrste Ixodes ricinus. Vrste iz porodice Anaplasmataceae je složeno uzgojiti na staničnim kulturama kao i dokazati u kliničkim uzorcima različitim bojanjima, stoga se u istraživanjima koriste molekularne metode. U ovom diplomskom radu analizirani su uzorci DNA izolirani iz krvi pasa arhive Laboratorija za parazitologiju Hratskog veterinarskog instituta. DNA je naprije umnožena lančanom reakcijom polimeraze „nested“ protokolom, a dijelovi 16S rRNA gena potom su sekvencionirani te analizirani odgovarajućim programima. Budući da ne postoje podaci o vrstama prisutnim u RH, cilj je bio istražiti koje su vrste iz roda Anaplasmataceae prisutne u pasa u Hrvatskoj te utvrditi učestalost pojedinih vrsta. Od ukupno 68 uzoraka pasa u njih 22 pronađena je DNA bakterija iz porodice Anaplasmataceae. Najzastupljenija je bila vrsta A. platys (64 %) a pronađene su i vrste A. phagocytophilum (18 %) te Wolbachia, endosimbiont Dirofilaria repens (18 %).

Abstract (english)

Anaplasmataceae is part of a family of small intracellular proteobacteria which includes the genera Anaplasma, Ehrlichia, Neorickettsia, Wolbachia, Aegyptianella and Candidatus Neoehrlihia. These bacteria survive as endosymbionts in other cells, but at the same time can cause diseases in humans and animals. Bacteria of the genus Anaplasma are transmitted by vectors (ticks), and the most important in Europe is the tick species Ixodes ricinus. Species of family Anaplasmataceae are complicated to grow in cell cultures as well as to prove them in clinical samples by different coloring, therefore the research is based on molecular methods. This thesis analyzed the samples of DNA isolated from the blood of dogs belonging to the archives of the Laboratory of parasitology of the Croatian veterinary institute. DNA was amplified by polymerase chain reaction "nested" protocol, 16S rRNA gene parts sequenced and analyzed in the appropriate programs. As there are no data on the species present in Croatia the goal was to investigate which species of the genus Anaplasmataceae are present in dogs from Croatia and to determine the frequency of certain species. From a total of 68 samples of dogs in 22 of them the DNA of bacteria from the family Anaplasmataceae was found. The most abundant species was A. Platys (64 %) and species A. phagocytophilum (18 %) and Wolbachia, endosimbiont of Dirofilaria repens (18 %) also were found.

• Keywords: unutarstanične bakterije; endosimbionti; Anaplasma platys; Anaplasma phagocytophilum; Wolbachia; 16S rRNA
• Keywords (english): intracellular bacteria; endosymbiont; Anaplasma platys; Anaplasma phagocytophilum; Wolbachia; 16S rRNA
• Language: croatian
• URN:NBN: urn:nbn:hr:217:382121
• Study programme: Title: Experimental Biology; Study programme type: university; Study level: graduate; Academic / professional title: magistar/magistra eksperimentalne biologije (magistar/magistra eksperimentalne biologije)
• Type of resource: Text
• File origin: Born digital
• Access conditions: Access restricted to students and staff of home institution
• Created on: 2017-04-26 11:19:27